Nephrin mRNA regulation by protein kinase C

Shixuan Wang, Paolo Mené, Harry Holthöfer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. Mutations in the recently cloned NPHS1 gene result in congenital nephrotic syndrome of the Finnish type (CNF). The protein product of NPHS1, nephrin, is expressed uniquely in kidney glomerular podocytes, and is the first true component of the interpodocyte slit membrane. The precise functions of nephrin remain unknown, but the presence of several tyrosine residues in the intracellular domain suggest a role in signalling. We searched for nephrin expressing cell line for use in signal transduction studies and also characterized the main features of calcium signalling in nephrin-deficient cultural glomerular epithelial cells. Methods. We used A293 cell line, found to naturally express nephrin, as well as cultured CNF glomerular cells using reverse-transcription PCR, immunocytochemistry and intracellular Ca2+ measurements. Results. Phorbol-12-myristate-13-acetate significantly upregulated the nephrin expression in A293 cells, while no change was found after treatment with additional stimulants for other main signalling pathways, e.g. okadaic acid, lysophosphatidic acid, bradykinin, angiotensin II (ANG II) and arginine vasopressin (AVP). No changes in basal or ANG II- or AVP-stimulated intracellular Ca2+ fluxes in CNF glomerular cells were observed. Conclusions. Protein kinase C may be the key intracellular signalling system in the regulation of nephrin.

Original languageEnglish (US)
Pages (from-to)98-103
Number of pages6
JournalJournal of Nephrology
Volume14
Issue number2
StatePublished - Mar 1 2001
Externally publishedYes

Fingerprint

Protein Kinase C
Messenger RNA
Arginine Vasopressin
Angiotensin II
Cell Line
Podocytes
Okadaic Acid
Calcium Signaling
Bradykinin
nephrin
Reverse Transcription
Tyrosine
Signal Transduction
Acetates
Epithelial Cells
Immunohistochemistry
Kidney
Polymerase Chain Reaction
Mutation
Membranes

Keywords

  • Ca
  • Nephrin
  • Protein kinase C

ASJC Scopus subject areas

  • Nephrology

Cite this

Wang, S., Mené, P., & Holthöfer, H. (2001). Nephrin mRNA regulation by protein kinase C. Journal of Nephrology, 14(2), 98-103.

Nephrin mRNA regulation by protein kinase C. / Wang, Shixuan; Mené, Paolo; Holthöfer, Harry.

In: Journal of Nephrology, Vol. 14, No. 2, 01.03.2001, p. 98-103.

Research output: Contribution to journalArticle

Wang, S, Mené, P & Holthöfer, H 2001, 'Nephrin mRNA regulation by protein kinase C', Journal of Nephrology, vol. 14, no. 2, pp. 98-103.
Wang S, Mené P, Holthöfer H. Nephrin mRNA regulation by protein kinase C. Journal of Nephrology. 2001 Mar 1;14(2):98-103.
Wang, Shixuan ; Mené, Paolo ; Holthöfer, Harry. / Nephrin mRNA regulation by protein kinase C. In: Journal of Nephrology. 2001 ; Vol. 14, No. 2. pp. 98-103.
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