Netrin-1: A Novel Universal Biomarker of Human Kidney Injury

G. Ramesh, O. Kwon, K. Ahn

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Currently available diagnostic markers representing kidney injury or function such as serum creatinine and blood urea nitrogen are insensitive and often increased late in the disease process. Netrin-1 protein, a laminin-related secreted molecule, is minimally or not expressed in tubular epithelial cells of normal kidneys. However, it is highly expressed in injured kidneys. Netrin-1 protein has been shown to be detected in urine from mice with acute kidney injury. The current study was carried out to evaluate whether netrin-1 is also induced in human acute kidney injury (AKI) and can serve as a urinary biomarker of the condition. We analyzed netrin-1 levels by sandwich enzyme-linked immunosorbent assay in urine samples from 10 healthy controls, 22 recipients of a renal allograft, 11 patients with ischemic AKI, 13 with AKI associated with sepsis, 9 with radiocontrast-induced AKI, and 8 with drug-induced AKI. Urinary netrin-1 levels normalized for urinary creatinine were significantly higher in all subject groups. The highest values were observed in patients with sepsis and in transplant patients immediately postoperatively. The level of NGAL was similarly increased in transplant patients. In conclusion, urinary netrin-1 levels are increased in patients with various forms of AKI/ATN and may serve as a universal biomarker for AKI.

Original languageEnglish (US)
Pages (from-to)1519-1522
Number of pages4
JournalTransplantation Proceedings
Volume42
Issue number5
DOIs
StatePublished - Jun 1 2010

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Acute Kidney Injury
Biomarkers
Kidney
Wounds and Injuries
Creatinine
Sepsis
Urine
Transplants
netrin-1
Blood Urea Nitrogen
Laminin
Allografts
Proteins
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Serum
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Netrin-1 : A Novel Universal Biomarker of Human Kidney Injury. / Ramesh, G.; Kwon, O.; Ahn, K.

In: Transplantation Proceedings, Vol. 42, No. 5, 01.06.2010, p. 1519-1522.

Research output: Contribution to journalArticle

Ramesh, G. ; Kwon, O. ; Ahn, K. / Netrin-1 : A Novel Universal Biomarker of Human Kidney Injury. In: Transplantation Proceedings. 2010 ; Vol. 42, No. 5. pp. 1519-1522.
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