TY - JOUR
T1 - Netrin-1, a urinary proximal tubular injury marker, is elevated early in the time course of human diabetes
AU - Jayakumar, Calpurnia
AU - Nauta, Ferdau L.
AU - Bakker, Stephan J.L.
AU - Bilo, Henk
AU - Gansevoort, Ron T.
AU - Johnson, Maribeth H.
AU - Ramesh, Ganesan
N1 - Funding Information:
Acknowledgments This work was supported by the National Institute of Health grant R01 grant (7R01DK083379-02) to GR.
PY - 2014/4
Y1 - 2014/4
N2 - Background: Netrin-1 was recently identified as an early diagnostic biomarker of chronic kidney disease (CKD) in an experimental animal model. However, its usefulness for early diagnosis of CKD in humans is unknown. The current study evaluated whether netrin-1 is increased in urine from human diabetic patients. Methods: Spot urine samples from healthy volunteers, diabetes without microalbuminuria, diabetes with microalbuminuria and diabetes with macroalbuminuria were collected after receiving consent. Netrin-1 in urine was quantified by enzyme-linked immunosorbent assay and the data analyzed to determine whether urinary netrin-1 significantly correlates with disease progression. Results: Urinary netrin-1 levels were significantly increased in normoalbuminuric diabetic patients compared to healthy controls, and still further elevated in patients with microalbuminuria and overt nephropathy. Urinary netrin-1 was significantly associated with albuminuria and estimated glomerular filtration rate, independently of age and sex. Conclusion: Netrin-1 is detectable in urine from diabetic patients and may serve as a useful early diagnostic biomarker predicting the development of CKD in diabetes.
AB - Background: Netrin-1 was recently identified as an early diagnostic biomarker of chronic kidney disease (CKD) in an experimental animal model. However, its usefulness for early diagnosis of CKD in humans is unknown. The current study evaluated whether netrin-1 is increased in urine from human diabetic patients. Methods: Spot urine samples from healthy volunteers, diabetes without microalbuminuria, diabetes with microalbuminuria and diabetes with macroalbuminuria were collected after receiving consent. Netrin-1 in urine was quantified by enzyme-linked immunosorbent assay and the data analyzed to determine whether urinary netrin-1 significantly correlates with disease progression. Results: Urinary netrin-1 levels were significantly increased in normoalbuminuric diabetic patients compared to healthy controls, and still further elevated in patients with microalbuminuria and overt nephropathy. Urinary netrin-1 was significantly associated with albuminuria and estimated glomerular filtration rate, independently of age and sex. Conclusion: Netrin-1 is detectable in urine from diabetic patients and may serve as a useful early diagnostic biomarker predicting the development of CKD in diabetes.
KW - Albuminuria
KW - Biomarker
KW - Diabetic nephropathy
KW - Netrin-1
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U2 - 10.1007/s40620-014-0055-2
DO - 10.1007/s40620-014-0055-2
M3 - Article
C2 - 24510764
AN - SCOPUS:84901006779
SN - 1121-8428
VL - 27
SP - 151
EP - 157
JO - Journal of Nephrology
JF - Journal of Nephrology
IS - 2
ER -
