Neuraminidase and contractile responses to norepinephrine in rat tail artery

J. H. Rice, R Clinton Webb

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Sialic acids are negatively charged groups in the carbohydrate side chains of glycolipids and glycoproteins which line the external membrane surface. The goal of this study was to characterize the effect of neuraminidase, which selectively cleaves sialic acids, on contractile activity in vascular smooth muscle. Helically cut strips of rat tail artery were mounted in an organ chamber and isometric contractions were recorded. Following treatment with neuraminidase (0.2 U/ml, 1 h), contractile responses to norepinephrine were signficantly greater than control responses. Phasic concentrations to norepinephrine in calcium-free medium were not altered by neuraminidase, whereas following calcium depletion with EGTA, contractile responses to added calcium were greater in enzyme-treated strips than in control when activated with norepinephrine. The augmentation of norepinephrine-induced contractions following neuraminidase treatment was reversed by incubation of the arterial strips with N-acetylneuraminic acid (10-4 M). Neuraminidase had no effect on contractile responses to potassium chloride, angiotensin II, and caffeine. Biochemical assay indicated that approximately 63% of the total sialic acid residues were removed from the arterial strips during incubation with the enzyme. It is concluded that a component for the control of the transmembrane calcium movement in response to norepinephrine is dependent on the presence of sialic acid residues.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalBlood Vessels
Volume21
Issue number1
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Neuraminidase
Tail
Norepinephrine
Arteries
N-Acetylneuraminic Acid
Sialic Acids
Calcium
Isometric Contraction
Potassium Chloride
Egtazic Acid
Glycolipids
Enzymes
Caffeine
Vascular Smooth Muscle
Angiotensin II
Glycoproteins
Carbohydrates
Membranes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Neuraminidase and contractile responses to norepinephrine in rat tail artery. / Rice, J. H.; Webb, R Clinton.

In: Blood Vessels, Vol. 21, No. 1, 01.01.1984, p. 1-11.

Research output: Contribution to journalArticle

@article{91c99e7e3f8c4a7ba6368146def58542,
title = "Neuraminidase and contractile responses to norepinephrine in rat tail artery",
abstract = "Sialic acids are negatively charged groups in the carbohydrate side chains of glycolipids and glycoproteins which line the external membrane surface. The goal of this study was to characterize the effect of neuraminidase, which selectively cleaves sialic acids, on contractile activity in vascular smooth muscle. Helically cut strips of rat tail artery were mounted in an organ chamber and isometric contractions were recorded. Following treatment with neuraminidase (0.2 U/ml, 1 h), contractile responses to norepinephrine were signficantly greater than control responses. Phasic concentrations to norepinephrine in calcium-free medium were not altered by neuraminidase, whereas following calcium depletion with EGTA, contractile responses to added calcium were greater in enzyme-treated strips than in control when activated with norepinephrine. The augmentation of norepinephrine-induced contractions following neuraminidase treatment was reversed by incubation of the arterial strips with N-acetylneuraminic acid (10-4 M). Neuraminidase had no effect on contractile responses to potassium chloride, angiotensin II, and caffeine. Biochemical assay indicated that approximately 63{\%} of the total sialic acid residues were removed from the arterial strips during incubation with the enzyme. It is concluded that a component for the control of the transmembrane calcium movement in response to norepinephrine is dependent on the presence of sialic acid residues.",
author = "Rice, {J. H.} and Webb, {R Clinton}",
year = "1984",
month = "1",
day = "1",
language = "English (US)",
volume = "21",
pages = "1--11",
journal = "Journal of Vascular Research",
issn = "1018-1172",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Neuraminidase and contractile responses to norepinephrine in rat tail artery

AU - Rice, J. H.

AU - Webb, R Clinton

PY - 1984/1/1

Y1 - 1984/1/1

N2 - Sialic acids are negatively charged groups in the carbohydrate side chains of glycolipids and glycoproteins which line the external membrane surface. The goal of this study was to characterize the effect of neuraminidase, which selectively cleaves sialic acids, on contractile activity in vascular smooth muscle. Helically cut strips of rat tail artery were mounted in an organ chamber and isometric contractions were recorded. Following treatment with neuraminidase (0.2 U/ml, 1 h), contractile responses to norepinephrine were signficantly greater than control responses. Phasic concentrations to norepinephrine in calcium-free medium were not altered by neuraminidase, whereas following calcium depletion with EGTA, contractile responses to added calcium were greater in enzyme-treated strips than in control when activated with norepinephrine. The augmentation of norepinephrine-induced contractions following neuraminidase treatment was reversed by incubation of the arterial strips with N-acetylneuraminic acid (10-4 M). Neuraminidase had no effect on contractile responses to potassium chloride, angiotensin II, and caffeine. Biochemical assay indicated that approximately 63% of the total sialic acid residues were removed from the arterial strips during incubation with the enzyme. It is concluded that a component for the control of the transmembrane calcium movement in response to norepinephrine is dependent on the presence of sialic acid residues.

AB - Sialic acids are negatively charged groups in the carbohydrate side chains of glycolipids and glycoproteins which line the external membrane surface. The goal of this study was to characterize the effect of neuraminidase, which selectively cleaves sialic acids, on contractile activity in vascular smooth muscle. Helically cut strips of rat tail artery were mounted in an organ chamber and isometric contractions were recorded. Following treatment with neuraminidase (0.2 U/ml, 1 h), contractile responses to norepinephrine were signficantly greater than control responses. Phasic concentrations to norepinephrine in calcium-free medium were not altered by neuraminidase, whereas following calcium depletion with EGTA, contractile responses to added calcium were greater in enzyme-treated strips than in control when activated with norepinephrine. The augmentation of norepinephrine-induced contractions following neuraminidase treatment was reversed by incubation of the arterial strips with N-acetylneuraminic acid (10-4 M). Neuraminidase had no effect on contractile responses to potassium chloride, angiotensin II, and caffeine. Biochemical assay indicated that approximately 63% of the total sialic acid residues were removed from the arterial strips during incubation with the enzyme. It is concluded that a component for the control of the transmembrane calcium movement in response to norepinephrine is dependent on the presence of sialic acid residues.

UR - http://www.scopus.com/inward/record.url?scp=0021366122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021366122&partnerID=8YFLogxK

M3 - Article

C2 - 6689956

AN - SCOPUS:0021366122

VL - 21

SP - 1

EP - 11

JO - Journal of Vascular Research

JF - Journal of Vascular Research

SN - 1018-1172

IS - 1

ER -