Neuregulin-increased expression of acetylcholine receptor ε-subunit gene requires ErbB interaction with Shc

Sandra Won, Jutong Si, Marcie Colledge, Kodimangalam S. Ravichandran, Stanley C. Froehner, Lin Mei

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Selective transcription of acetylcholine receptor (AChR) subunit genes by neuregulin is one of the mechanisms involved in the synaptic localization of AChRs to the neuromuscular junction. Neuregulin stimulates ErbB receptor tyrosine kinases and subsequently activates the Ras/ERK pathway, which is required for neuregulin-mediated induction of AChR subunit genes in muscle cells and synapse-specific expression in vivo. Here we investigated the neuregulin transduction mechanism that leads to ERK activation after ErbB receptor tyrosine phosphorylation. Neuregulin increases the association of the adaptor proteins Grb2 and Shc with both ErbB2 and ErbB3 in C2C12 muscle cells. Dephosphorylation of the tyrosine-phosphorylated ErbB proteins abolished their association with both Grb2 and Shc, suggesting a tyrosine phosphorylation-dependent interaction. The interaction of Shc with the ErbB receptors is mediated by Shc's phosphotyrosine-binding domain. In addition, neuregulin increased tyrosine phosphorylation of Shc. Mutagenesis approaches demonstrated that tyrosine phosphorylation of Shc is required for neuregulin induction of AChR subunit gene expression. Taken together, these data indicate that the interaction of ErbB receptors with Grb2 alone is insufficient for neuregulin-activated transcription, but that ErbB receptor signaling via Shc is necessary and important.

Original languageEnglish (US)
Pages (from-to)2358-2368
Number of pages11
JournalJournal of Neurochemistry
Volume73
Issue number6
DOIs
StatePublished - Nov 29 1999

Fingerprint

erbB Genes
Neuregulins
Cholinergic Receptors
Genes
Phosphorylation
Tyrosine
Transcription
Muscle Cells
Muscle
Shc Signaling Adaptor Proteins
Cells
Association reactions
Mutagenesis
Phosphotyrosine
MAP Kinase Signaling System
Neuromuscular Junction
Gene expression
Protein-Tyrosine Kinases
Synapses
Proteins

Keywords

  • Acetylcholine receptor
  • ErbB
  • Grb2
  • Neuregulin
  • Neuromuscular junction
  • Shc
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neuregulin-increased expression of acetylcholine receptor ε-subunit gene requires ErbB interaction with Shc. / Won, Sandra; Si, Jutong; Colledge, Marcie; Ravichandran, Kodimangalam S.; Froehner, Stanley C.; Mei, Lin.

In: Journal of Neurochemistry, Vol. 73, No. 6, 29.11.1999, p. 2358-2368.

Research output: Contribution to journalArticle

Won, Sandra ; Si, Jutong ; Colledge, Marcie ; Ravichandran, Kodimangalam S. ; Froehner, Stanley C. ; Mei, Lin. / Neuregulin-increased expression of acetylcholine receptor ε-subunit gene requires ErbB interaction with Shc. In: Journal of Neurochemistry. 1999 ; Vol. 73, No. 6. pp. 2358-2368.
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