Neuroendocrine peptides stimulate adenyl cyclase in normal and malignant prostate cells

Peter J. Gkonos, Balakrishna L. Lokeshwar, Wayne Balkan, Bernard A. Roos

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Elevations of intracellular cAMP in human prostate cancer cells have been shown to increase invasiveness and to promote neuronal differentiation. since neuroendocrine peptides capable of activating adenyl cyclase are present in prostatic nerves and epithelial neuroendocrine cells, we investigated normal and malignant human prostate cells for changes in intracellular cAMP in response to the prostatic peptides vasoactive intestinal peptide (VIP), calcitonin (CT), and calcitonin gene-related peptide (CGRP). Normal prostate epithelial cells and LNCaP prostate cancer cells exhibited, respectively, 6-and 30-fold increases in intracellular cAMP in response to VIP. ALVA-31 and PPC-1 prostate cancer cells demonstrated 20- to 200-fold increases in cAMP in response to CGRP, while normal epithelial cells and LNCaP cells exhibited smaller (2- to 6-fold) responses. Only DU-145 cells increased cAMP substantially in response to CT. VIP receptor mRNA was identified by Northern blot analysis only in those cells that responded to VIP. CT receptor mRNA was identified only in DU-145 cells by polymerase chain reaction and Southern blot analysis. These results suggest that VIP and possibly CGRP receptors are likely to be present in both normal and malignant prostate cells. VIP or CGRP may regulate secretion of proteases by normal or prostate cancer cells and may influence epithelial cell differentiation.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalRegulatory Peptides
Volume59
Issue number1
DOIs
StatePublished - Sep 22 1995

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Vasoactive Intestinal Peptide
Adenylyl Cyclases
Prostate
Calcitonin Gene-Related Peptide
Peptides
Cells
Calcitonin
Prostatic Neoplasms
Epithelial Cells
Calcitonin Receptors
Vasoactive Intestinal Peptide Receptors
Calcitonin Gene-Related Peptide Receptors
Messenger RNA
Polymerase chain reaction
Peptide Hydrolases
Neuroendocrine Cells
Southern Blotting
Northern Blotting
Cell Differentiation
Polymerase Chain Reaction

Keywords

  • Calcitonin
  • Calcitonin gene-related peptide
  • Prostate
  • Receptor
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neuroendocrine peptides stimulate adenyl cyclase in normal and malignant prostate cells. / Gkonos, Peter J.; Lokeshwar, Balakrishna L.; Balkan, Wayne; Roos, Bernard A.

In: Regulatory Peptides, Vol. 59, No. 1, 22.09.1995, p. 43-51.

Research output: Contribution to journalArticle

Gkonos, Peter J. ; Lokeshwar, Balakrishna L. ; Balkan, Wayne ; Roos, Bernard A. / Neuroendocrine peptides stimulate adenyl cyclase in normal and malignant prostate cells. In: Regulatory Peptides. 1995 ; Vol. 59, No. 1. pp. 43-51.
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