Neuronal differentiation of cryopreserved neural progenitor cells derived from mouse embryonic stem cells

Christopher R. Hancock, Jonathon P. Wetherington, Nevin A. Lambert, Brian G. Condie

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Embryonic stem cells (ES cells) are developmentally pluripotent cells isolated from pre-implantation mammalian embryos. In cell culture ES cells can be easily differentiated to generate cultures of neural progenitors. We present a simple method for the cryopreservation of these ES-derived neural progenitors. Cryopreserved neural progenitor stocks can be thawed, expanded with FGF2, and differentiated into functional neurons. This method will facilitate studies using ES-derived neural progenitor cells as a cell culture model system for neural development and differentiation. It will also aid studies designed to test the ability of these progenitor cells to functionally engraft and repair damaged neural tissue. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)418-421
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume271
Issue number2
DOIs
StatePublished - May 10 2000

Keywords

  • Cell culture
  • Cryopreservation
  • Embryonic stem cells
  • Neural stem cells
  • Neuronal differentiation
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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