Neuronal growth-inhibitory factor (metallothionein-3): Structure-function relationships

Zhi Chun Ding; Feng Yun Ni; Zhong Xian Huang

doi:10.1111/j.1742-4658.2010.07716.x

Neuronal growth-inhibitory factor (metallothionein-3): Structure-function relationships

Zhi Chun Ding, Feng Yun Ni, Zhong Xian Huang

Research output: Contribution to journal › Short survey › peer-review

31 Scopus citations

Abstract

Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.

Original language	English (US)
Pages (from-to)	2912-2920
Number of pages	9
Journal	FEBS Journal
Volume	277
Issue number	14
DOIs	https://doi.org/10.1111/j.1742-4658.2010.07716.x
State	Published - Jul 2010
Externally published	Yes

Keywords

metallothionein (MT)
mutation
neuronal growth-inhibitory factor (GIF)
structure-function relationship

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1111/j.1742-4658.2010.07716.x

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title = "Neuronal growth-inhibitory factor (metallothionein-3): Structure-function relationships",

abstract = "Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.",

keywords = "metallothionein (MT), mutation, neuronal growth-inhibitory factor (GIF), structure-function relationship",

author = "Ding, {Zhi Chun} and Ni, {Feng Yun} and Huang, {Zhong Xian}",

year = "2010",

month = jul,

doi = "10.1111/j.1742-4658.2010.07716.x",

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journal = "FEBS Journal",

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T1 - Neuronal growth-inhibitory factor (metallothionein-3)

T2 - Structure-function relationships

AU - Ding, Zhi Chun

AU - Ni, Feng Yun

AU - Huang, Zhong Xian

PY - 2010/7

Y1 - 2010/7

N2 - Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.

AB - Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.

KW - metallothionein (MT)

KW - mutation

KW - neuronal growth-inhibitory factor (GIF)

KW - structure-function relationship

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DO - 10.1111/j.1742-4658.2010.07716.x

M3 - Short survey

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AN - SCOPUS:77954355688

SN - 1742-464X

VL - 277

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JO - FEBS Journal

JF - FEBS Journal

IS - 14

ER -

Neuronal growth-inhibitory factor (metallothionein-3): Structure-function relationships

Abstract

Keywords

ASJC Scopus subject areas

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