Neuronal growth-inhibitory factor (metallothionein-3): Structure-function relationships

Zhi-Chun Ding, Feng Yun Ni, Zhong Xian Huang

Research output: Contribution to journalShort survey

24 Citations (Scopus)

Abstract

Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.

Original languageEnglish (US)
Pages (from-to)2912-2920
Number of pages9
JournalFEBS Journal
Volume277
Issue number14
DOIs
StatePublished - Jul 1 2010

Fingerprint

Mutant Proteins
Molecular Dynamics Simulation
Bioactivity
Conformations
Molecular dynamics
Metals
Nuclear magnetic resonance
growth inhibitory factor
Computer simulation
Neuronal Outgrowth

Keywords

  • metallothionein (MT)
  • mutation
  • neuronal growth-inhibitory factor (GIF)
  • structure-function relationship

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Neuronal growth-inhibitory factor (metallothionein-3) : Structure-function relationships. / Ding, Zhi-Chun; Ni, Feng Yun; Huang, Zhong Xian.

In: FEBS Journal, Vol. 277, No. 14, 01.07.2010, p. 2912-2920.

Research output: Contribution to journalShort survey

Ding, Zhi-Chun ; Ni, Feng Yun ; Huang, Zhong Xian. / Neuronal growth-inhibitory factor (metallothionein-3) : Structure-function relationships. In: FEBS Journal. 2010 ; Vol. 277, No. 14. pp. 2912-2920.
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