Abstract
Spreading depolarization-induced focal dendritic swelling (beading) is an early hallmark of neuronal cytotoxic edema. Pyramidal neurons lack membrane-bound aquaporins posing a question of how water enters neurons during spreading depolarization. Recently, we have identified chloride-coupled transport mechanisms that can, at least in part, participate in dendritic beading. Yet transporter-mediated ion and water fluxes could be paralleled by water entry through additional pathways such as large-pore pannexin-1 channels opened by spreading depolarization. Using real-time in vivo two-photon imaging in mice with pharmacological inhibition or conditional genetic deletion of pannexin-1, we showed that pannexin-1 channels are not required for spreading depolarization-induced focal dendritic swelling.
Original language | English (US) |
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Pages (from-to) | 1626-1633 |
Number of pages | 8 |
Journal | Journal of Cerebral Blood Flow and Metabolism |
Volume | 37 |
Issue number | 5 |
DOIs | |
State | Published - May 1 2017 |
Keywords
- Conditional pannexin-1 knock-out
- mefloquine
- neuronal swelling
- two-photon imaging
- viral vectors
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
- Cardiology and Cardiovascular Medicine