Neurons and astroglia express distinct subsets of Na,K-ATPase α and β subunits

Richard S Cameron, Laura Klein, Andrew W. Shyjan, Pasko Rakic, Robert Levenson

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

We have analyzed the expression pattern of Na,K-ATPase α and β subunit isoforms within the rodent and primate central nervous system. Membrane fractions prepared from rat cerebral cortical type-1 astrocytes and rat cerebellar granule and hippocampal neurons were characterized by immunoblot analyses using a panel of α and β subunit isoform-specific antisera. Each cell type was found to express the α1 isoform but showed differences in the expression of other subunits. Cortical astrocytes displayed α2 and β2 subunits, whereas cerebellar granule neurons showed expression of α3 and β1 subunits. All three α subunit isotypes were detected in hippocampal neurons. A survey of the immunofluorescent staining pattern of the α3 subunit in rat and monkey brain confirmed that expression of this Na,K-ATPase α subunit isoform was restricted exclusively to neurons. These results suggest that both neurons and astrocytes express multiple, yet distinct, Na,K-ATPase isoenzymes. The identification of cell types expressing limited combinations of α and β subunits should provide a framework for understanding the physiological significance of Na,K-ATPase isoenzyme diversity and may provide useful tools for the analysis of cell lineage in the mammalian central nervous system.

Original languageEnglish (US)
Pages (from-to)333-343
Number of pages11
JournalMolecular Brain Research
Volume21
Issue number3-4
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Astrocytes
Protein Isoforms
Neurons
Isoenzymes
Central Nervous System
Cell Lineage
Primates
Haplorhini
Immune Sera
Rodentia
sodium-translocating ATPase
Staining and Labeling
Membranes
Brain

Keywords

  • Astrocyte
  • Granule cell
  • Immunoblotting
  • Immunocytochemistry
  • Monkey brain
  • Rat brain
  • Sodium pump isoform

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Neurons and astroglia express distinct subsets of Na,K-ATPase α and β subunits. / Cameron, Richard S; Klein, Laura; Shyjan, Andrew W.; Rakic, Pasko; Levenson, Robert.

In: Molecular Brain Research, Vol. 21, No. 3-4, 01.01.1994, p. 333-343.

Research output: Contribution to journalArticle

Cameron, Richard S ; Klein, Laura ; Shyjan, Andrew W. ; Rakic, Pasko ; Levenson, Robert. / Neurons and astroglia express distinct subsets of Na,K-ATPase α and β subunits. In: Molecular Brain Research. 1994 ; Vol. 21, No. 3-4. pp. 333-343.
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