Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats

Muzamil Ahmad, Sofiyan Saleem, Abdullah Shafique Ahmad, Mubeen Ahmad Ansari, Seema Yousuf, Md Nasrul Hoda, Fakhrul Islam

Research output: Contribution to journalArticle

140 Scopus citations

Abstract

6-Hydroxydopamine (6-OHDA) is one of the most widely used rat models for Parkinson's disease. There is ample evidence in the literature that 6-OHDA elicits its toxic manifestations through oxidant stress. In the present study, we evaluated the anti-parkinsonian effects of Withania somnifera extract, which has been reported to have potent anti-oxidant, anti-peroxidative and free radical quenching properties in various diseased conditions. Rats were pretreated with 100, 200 and 300 mg/kg b.w. of the W. somnifera extract orally for 3 weeks. On day 21, 2 μL of 6-OHDA (10 μg in 0.1% in ascorbic acid-saline) was infused into the right striatum while sham operated group received 2 μL of the vehicle. Three weeks after 6-OHDA injections, rats were tested for neurobehavioral activity and were killed 5 weeks after lesioning for the estimation of lipidperoxidation, reduced glutathione content, activities of glutathione-S-transferase, glutathione reductase, glutathione peroxidase, superoxide dismutase and catalase, catecholamine content, dopaminergic D 2 receptor binding and tyrosine hydroxylase expression. W. somnifera extract was found to reverse all the parameters significantly in a dose-dependent manner. Thus, the study demonstrates that the extract of W. somnifera may be helpful in protecting the neuronal injury in Parkinson's disease.

Original languageEnglish (US)
Pages (from-to)137-147
Number of pages11
JournalHuman and Experimental Toxicology
Volume24
Issue number3
DOIs
StatePublished - Mar 1 2005

    Fingerprint

Keywords

  • 6-hydroxydopamine
  • Antioxidant
  • Dopamine
  • Oxidative stress
  • Parkinson's disease
  • Withania somnifera

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Ahmad, M., Saleem, S., Ahmad, A. S., Ansari, M. A., Yousuf, S., Hoda, M. N., & Islam, F. (2005). Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats. Human and Experimental Toxicology, 24(3), 137-147. https://doi.org/10.1191/0960327105ht509oa