Neuroprotective role of erythropoietin by antiapoptosis in the retina

Hyewon Chung, Hyunju Lee, Folami Lamoke, William J.M. Hrushesky, Patricia A. Wood, Wan Jin Jahng

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Erythropoietin (EPO) stimulates red blood cell production, in part by inhibiting apoptosis of the red blood cell precursors. The erythropoietic effects of EPO are circadian stage dependent. Retinal injury due to light occurs through oxidative mechanisms and is manifest by retinal and retinal pigment epithelium (RPE) cells apoptosis. The visual cycle might be circadian coordinated as a means of effectively protecting the retina from the detrimental effects of light-induced, oxygen-dependent, free radical-mediated damage, especially at the times of day when light is more intense. We show that the retinal expression of EPO and its receptor (EPOR), as well as subsequent Janus kinase 2 (Jak2) phosphorylations, are each tightly linked to a specific time after oxidative stress and in anticipation of daily light onset. This is consistent with physiological protection against daily light-induced, oxidatively mediated retinal apoptosis. In vitro, we verify that EPO protects RPE cells from light, hyperoxia, and hydrogen peroxide-induced retinal cell apoptosis, and that these stimuli increase EPO and EPOR expression in cultured RPE cells. Together, these data support the premise that EPO and its EPOR interactions represent an important retinal shield from physiologic and pathologic light-induced oxidative injury.

Original languageEnglish (US)
Pages (from-to)2365-2374
Number of pages10
JournalJournal of Neuroscience Research
Volume87
Issue number10
DOIs
StatePublished - Aug 1 2009

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Keywords

  • Erythropoietin
  • Neuroprotection
  • Oxidative stress
  • Retina
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Chung, H., Lee, H., Lamoke, F., Hrushesky, W. J. M., Wood, P. A., & Jahng, W. J. (2009). Neuroprotective role of erythropoietin by antiapoptosis in the retina. Journal of Neuroscience Research, 87(10), 2365-2374. https://doi.org/10.1002/jnr.22046