Next generation sequencing: Advances in characterizing the Methylome

Kristen H. Taylor, Huidong Shi, Charles W. Caldwell

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Epigenetic modifications play an important role in lymphoid malignancies. This has been evidenced by the large body of work published using microarray technologies to generate methylation profiles for numerous types and subtypes of lymphoma and leukemia. These studies have shown the importance of defining the epigenome so that we can better understand the biology of lymphoma. Recent advances in DNA sequencing technology have transformed the landscape of epigenomic analysis as we now have the ability to characterize the genome-wide distribution of chromatin modifications and DNA methylation using next-generation sequencing. To take full advantage of the throughput of next-generation sequencing, there are many methodologies that have been developed and many more that are currently being developed. Choosing the appropriate methodology is fundamental to the outcome of next-generation sequencing studies. In this review, published technologies and methodologies applicable to studying the methylome are presented. In addition, progress towards defining the methylome in lymphoma is discussed and prospective directions that have been made possible as a result of next-generation sequencing technology. Finally, methodologies are introduced that have not yet been published but that are being explored in the pursuit of defining the lymphoma methylome.

Original languageEnglish (US)
Pages (from-to)143-165
Number of pages23
JournalGenes
Volume1
Issue number2
DOIs
StatePublished - Sep 1 2010

Fingerprint

Lymphoma
Technology
Epigenomics
DNA Methylation
DNA Sequence Analysis
Methylation
Chromatin
Leukemia
Genome
Neoplasms

Keywords

  • Epigenome
  • Leukemia
  • Lymphoma
  • Methylation
  • Next-generation sequencing

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Next generation sequencing : Advances in characterizing the Methylome. / Taylor, Kristen H.; Shi, Huidong; Caldwell, Charles W.

In: Genes, Vol. 1, No. 2, 01.09.2010, p. 143-165.

Research output: Contribution to journalReview article

Taylor, Kristen H. ; Shi, Huidong ; Caldwell, Charles W. / Next generation sequencing : Advances in characterizing the Methylome. In: Genes. 2010 ; Vol. 1, No. 2. pp. 143-165.
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