Next-generation sequencing for viruses in children with rapid-onset type 1 diabetes

H. S. Lee, T. Briese, C. Winkler, M. Rewers, E. Bonifacio, H. Hyoty, M. Pflueger, O. Simell, J. X. She, W. Hagopian, A. Lernmark, B. Akolkar, J. P. Krischer, A. G. Ziegler

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Aims/hypothesis: Viruses are candidate causative agents in the pathogenesis of autoimmune (type 1) diabetes. We hypothesised that children with a rapid onset of type 1 diabetes may have been exposed to such agents shortly before the initiation of islet autoimmunity, possibly at high dose, and thus study of these children could help identify viruses involved in the development of autoimmune diabetes. Methods: We used next-generation sequencing to search for viruses in plasma samples and examined the history of infection and fever in children enrolled in The Environmental Determinants of Diabetes in the Young (TEDDY) study who progressed to type 1 diabetes within 6 months from the appearance of islet autoimmunity, and in matched islet-autoantibody-negative controls. Results: Viruses were not detected more frequently in plasma from rapid-onset patients than in controls during the period surrounding seroconversion. In addition, infection histories were found to be similar between children with rapid-onset diabetes and control children, although episodes of fever were reported less frequently in children with rapid-onset diabetes. Conclusions/interpretation: These findings do not support the presence of viraemia around the time of seroconversion in young children with rapid-onset type 1 diabetes.

Original languageEnglish (US)
Pages (from-to)1705-1711
Number of pages7
JournalDiabetologia
Volume56
Issue number8
DOIs
StatePublished - Aug 2013
Externally publishedYes

Keywords

  • GB virus C
  • Human herpes virus 6
  • Human rhinovirus C
  • Infections
  • Islet autoimmunity
  • Next-generation deep sequencing
  • Parvovirus B19
  • TEDDY study
  • Type 1 diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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