NF-κB is required for TNF-α-directed smooth muscle cell migration

Zhongbiao Wang, Manuel R. Castresana, Walter H. Newman

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Migration of vascular smooth muscle cells (VSMC) is a crucial event in the formation of vascular stenotic lesions. Tumor necrosis factor-α (TNF-α) is elaborated by VSMC in atherosclerosis and following angioplasty. We investigated the role of nuclear factor-κB (NF-κB) in human VSMC migration induced by TNF-α. Adenoviral expression of a mutant form of the inhibitor of NF-κB, IκB-αM, suppressed TNF-α-triggered degradation of cellular IκB-α, inhibited activation of NF-κB, and attenuated TNF-α-induced migration. Further, IκB-αM suppressed TNF-α-stimulated release of interleukin-6 and -8 (IL-6 and IL-8). Neutralization of IL-6 and IL-8 with appropriate antibodies reduced TNF-α-induced VSMC migration. Addition of recombinant IL-6 and IL-8 stimulated migration. Collectively, our data provide initial evidence that TNF-α-mediated VSMC migration requires NF-κB activation and is associated with induction of IL-6 and IL-8 which act in an autocrine manner.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalFEBS Letters
Volume508
Issue number3
DOIs
StatePublished - Nov 23 2001
Externally publishedYes

Keywords

  • Interleukin-6
  • Interleukin-8
  • Migration
  • Nuclear factor κB
  • Smooth muscle cell
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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