Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development

N. M. Fletcher, S. Abuanzeh, M. G. Saed, M. P. Diamond, H. M. Abu-Soud, Ghassan M. Saed

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have previously reported that superoxide (O2•-) contributes to the development of postoperative adhesions. In this study, we determined whether O2•- generating nicotinamide adenine dinucleotide phosphate oxidase (NOX) is differentially expressed in normal peritoneal and adhesion fibroblasts and tissues. The NOX isoforms were measured utilizing Western blot, immunohistochemistry, high-performance liquid chromatography, and real-time reverse transcription polymerase chain reaction. Expression and activity of NOX were found to be significantly higher in adhesion tissues and cells than that in normal peritoneal tissues and cells (P <.05). Levels of NOX2, NOX4, NOX activating protein 1, DUOX1, p47phox, and p22phox messenger RNA increased in adhesion fibroblasts when compared to normal peritoneal and increased in response to hypoxia in normal peritoneal fibroblasts. Thus, adhesion fibroblasts are characterized by a unique NOX expression profile, which maintains a pro-oxidant state that may be responsible for the persistence of the adhesion phenotype. Decreasing the activity of NOX by targeting these isoforms may be beneficial for future therapeutic interventions of postoperative adhesions.

Original languageEnglish (US)
Pages (from-to)1050-1059
Number of pages10
JournalReproductive Sciences
Volume21
Issue number8
DOIs
StatePublished - Aug 2014

Fingerprint

NADP
Reactive Oxygen Species
Oxidoreductases
Fibroblasts
Tissue Adhesions
Protein Isoforms
Cell Adhesion
Superoxides
Reverse Transcription
Western Blotting
Immunohistochemistry
High Pressure Liquid Chromatography
Phenotype
Polymerase Chain Reaction
Messenger RNA
Proteins
Therapeutics

Keywords

  • NADPH oxidase
  • adhesions
  • fibroblasts
  • hypoxia
  • oxidative stress

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development. / Fletcher, N. M.; Abuanzeh, S.; Saed, M. G.; Diamond, M. P.; Abu-Soud, H. M.; Saed, Ghassan M.

In: Reproductive Sciences, Vol. 21, No. 8, 08.2014, p. 1050-1059.

Research output: Contribution to journalArticle

@article{82302e15fdb14f4aaf34f06b61e50f6b,
title = "Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development",
abstract = "We have previously reported that superoxide (O2•-) contributes to the development of postoperative adhesions. In this study, we determined whether O2•- generating nicotinamide adenine dinucleotide phosphate oxidase (NOX) is differentially expressed in normal peritoneal and adhesion fibroblasts and tissues. The NOX isoforms were measured utilizing Western blot, immunohistochemistry, high-performance liquid chromatography, and real-time reverse transcription polymerase chain reaction. Expression and activity of NOX were found to be significantly higher in adhesion tissues and cells than that in normal peritoneal tissues and cells (P <.05). Levels of NOX2, NOX4, NOX activating protein 1, DUOX1, p47phox, and p22phox messenger RNA increased in adhesion fibroblasts when compared to normal peritoneal and increased in response to hypoxia in normal peritoneal fibroblasts. Thus, adhesion fibroblasts are characterized by a unique NOX expression profile, which maintains a pro-oxidant state that may be responsible for the persistence of the adhesion phenotype. Decreasing the activity of NOX by targeting these isoforms may be beneficial for future therapeutic interventions of postoperative adhesions.",
keywords = "NADPH oxidase, adhesions, fibroblasts, hypoxia, oxidative stress",
author = "Fletcher, {N. M.} and S. Abuanzeh and Saed, {M. G.} and Diamond, {M. P.} and Abu-Soud, {H. M.} and Saed, {Ghassan M.}",
year = "2014",
month = "8",
doi = "10.1177/1933719114522524",
language = "English (US)",
volume = "21",
pages = "1050--1059",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "8",

}

TY - JOUR

T1 - Nicotinamide adenine dinucleotide phosphate oxidase expression is differentially regulated to favor a pro-oxidant state that contributes to postoperative adhesion development

AU - Fletcher, N. M.

AU - Abuanzeh, S.

AU - Saed, M. G.

AU - Diamond, M. P.

AU - Abu-Soud, H. M.

AU - Saed, Ghassan M.

PY - 2014/8

Y1 - 2014/8

N2 - We have previously reported that superoxide (O2•-) contributes to the development of postoperative adhesions. In this study, we determined whether O2•- generating nicotinamide adenine dinucleotide phosphate oxidase (NOX) is differentially expressed in normal peritoneal and adhesion fibroblasts and tissues. The NOX isoforms were measured utilizing Western blot, immunohistochemistry, high-performance liquid chromatography, and real-time reverse transcription polymerase chain reaction. Expression and activity of NOX were found to be significantly higher in adhesion tissues and cells than that in normal peritoneal tissues and cells (P <.05). Levels of NOX2, NOX4, NOX activating protein 1, DUOX1, p47phox, and p22phox messenger RNA increased in adhesion fibroblasts when compared to normal peritoneal and increased in response to hypoxia in normal peritoneal fibroblasts. Thus, adhesion fibroblasts are characterized by a unique NOX expression profile, which maintains a pro-oxidant state that may be responsible for the persistence of the adhesion phenotype. Decreasing the activity of NOX by targeting these isoforms may be beneficial for future therapeutic interventions of postoperative adhesions.

AB - We have previously reported that superoxide (O2•-) contributes to the development of postoperative adhesions. In this study, we determined whether O2•- generating nicotinamide adenine dinucleotide phosphate oxidase (NOX) is differentially expressed in normal peritoneal and adhesion fibroblasts and tissues. The NOX isoforms were measured utilizing Western blot, immunohistochemistry, high-performance liquid chromatography, and real-time reverse transcription polymerase chain reaction. Expression and activity of NOX were found to be significantly higher in adhesion tissues and cells than that in normal peritoneal tissues and cells (P <.05). Levels of NOX2, NOX4, NOX activating protein 1, DUOX1, p47phox, and p22phox messenger RNA increased in adhesion fibroblasts when compared to normal peritoneal and increased in response to hypoxia in normal peritoneal fibroblasts. Thus, adhesion fibroblasts are characterized by a unique NOX expression profile, which maintains a pro-oxidant state that may be responsible for the persistence of the adhesion phenotype. Decreasing the activity of NOX by targeting these isoforms may be beneficial for future therapeutic interventions of postoperative adhesions.

KW - NADPH oxidase

KW - adhesions

KW - fibroblasts

KW - hypoxia

KW - oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=84905818205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905818205&partnerID=8YFLogxK

U2 - 10.1177/1933719114522524

DO - 10.1177/1933719114522524

M3 - Article

AN - SCOPUS:84905818205

VL - 21

SP - 1050

EP - 1059

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 8

ER -