Nicotinamide reduces acute cortical neuronal death and edema in the traumatically injured brain

Michael R. Hoane, David R. Gilbert, Michael A. Holland, Jeremy L. Pierce

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Previous studies have shown that administration of nicotinamide (Vitamin B3) in animal models of traumatic brain injury (TBI) and ischemia significantly reduced the size of infarction or injury and improved functional recovery. The present study evaluated the ability of nicotinamide to provide acute neuroprotection and edema reduction following TBI. Groups of rats were assigned to nicotinamide (500 mg/kg) or saline (1.0 ml/kg) treatment conditions and received contusion injuries or sham surgeries. Drug treatment was administered 15 min following injury. Brains were harvested 24 h later and either processed for histology or water content. Frozen sections were stained with the degenerating neuron stain (Fluoro-Jade® B) (FJ) and cell counts were performed at the site of injury. Additional brains were processed for water content (a measure of injury-induced edema). Results of this study showed that administration of nicotinamide following TBI significantly reduced the number of FJ+ neurons in the injured cortex compared to saline-treated animals. Examination of the water content of the brains also revealed that administration of nicotinamide significantly attenuated the amount of water compared to saline-treated animals in the injured cortex. These results indicate that nicotinamide administration significantly reduced neuronal death and attenuated cerebral edema following injury. The current findings suggest that nicotinamide significantly modulates acute pathophysiological processes following injury and that this may account for its beneficial effects on recovery of function following injury.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalNeuroscience Letters
Volume408
Issue number1
DOIs
StatePublished - Nov 6 2006
Externally publishedYes

Fingerprint

Niacinamide
Edema
Wounds and Injuries
Brain
Water
Neurons
Contusions
Recovery of Function
Brain Edema
Frozen Sections
Brain Ischemia
Infarction
Histology
B-Lymphocytes
Coloring Agents
Animal Models
Cell Count
Therapeutics
Pharmaceutical Preparations

Keywords

  • Edema
  • Fluoro-jade
  • Neuroprotection
  • Nicotinamide
  • Rat
  • TBI
  • Vitamin B

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nicotinamide reduces acute cortical neuronal death and edema in the traumatically injured brain. / Hoane, Michael R.; Gilbert, David R.; Holland, Michael A.; Pierce, Jeremy L.

In: Neuroscience Letters, Vol. 408, No. 1, 06.11.2006, p. 35-39.

Research output: Contribution to journalArticle

Hoane, Michael R. ; Gilbert, David R. ; Holland, Michael A. ; Pierce, Jeremy L. / Nicotinamide reduces acute cortical neuronal death and edema in the traumatically injured brain. In: Neuroscience Letters. 2006 ; Vol. 408, No. 1. pp. 35-39.
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