Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation

Meiling Zheng, Juan Cai, Zhiwen Liu, Shaoqun Shu, Ying Wang, Chengyuan Tang, Zheng Dong

Research output: Contribution to journalArticle

Abstract

Renal interstitial fibrosis is a common pathological feature in progressive kidney diseases currently lacking effective treatment. Nicotinamide (NAM), a member of water-soluble vitamin B family, was recently suggested to have a therapeutic potential for acute kidney injury (AKI) in mice and humans. The effect of NAM on chronic kidney pathologies, including renal fibrosis, is unknown. Here we have tested the effects of NAM on renal interstitial fibrosis using in vivo and in vitro models. In vivo, unilateral urethral obstruction (UUO) induced renal interstitial fibrosis as indicated Masson trichrome staining and expression of pro-fibrotic proteins, which was inhibited by NAM. In UUO, NAM suppressed tubular atrophy and apoptosis. In addition, NAM suppressed UUO-associated T cell and macrophage infiltration and induction of pro-inflammatory cytokines, such as TNF-α and IL-1β. In cultured mouse proximal tubule cells, NAM blocked TGF–β-induced expression of fibrotic proteins, while it marginally suppressed the morphological changes induced by TGF-β. NAM also suppressed the expression of pro-inflammatory cytokines (eg MCP-1 and IL-1β) during TGF-β treatment of these cells. Collectively, the results demonstrate an anti-fibrotic effect of NAM in kidneys, which may involve the suppression of tubular injury and inflammation.

Original languageEnglish (US)
Pages (from-to)3995-4004
Number of pages10
JournalJournal of Cellular and Molecular Medicine
Volume23
Issue number6
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

Fingerprint

Niacinamide
Fibrosis
Inflammation
Kidney
Wounds and Injuries
Urethral Obstruction
Interleukin-1
Cytokines
Vitamin B Complex
Kidney Diseases
Acute Kidney Injury
Atrophy
Proteins
Macrophages
Apoptosis
Pathology
Staining and Labeling
T-Lymphocytes
Water

Keywords

  • NAD
  • apoptosis
  • inflammation
  • renal fibrosis
  • tubular atrophy

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation. / Zheng, Meiling; Cai, Juan; Liu, Zhiwen; Shu, Shaoqun; Wang, Ying; Tang, Chengyuan; Dong, Zheng.

In: Journal of Cellular and Molecular Medicine, Vol. 23, No. 6, 01.06.2019, p. 3995-4004.

Research output: Contribution to journalArticle

Zheng, Meiling ; Cai, Juan ; Liu, Zhiwen ; Shu, Shaoqun ; Wang, Ying ; Tang, Chengyuan ; Dong, Zheng. / Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation. In: Journal of Cellular and Molecular Medicine. 2019 ; Vol. 23, No. 6. pp. 3995-4004.
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