Nicotinic acid inhibits glucose-stimulated insulin secretion via the G protein-coupled receptor PUMA-G in murine islet β cells

Hong Ming Li, Mei Zhang, Sheng Tao Xu, Di Zheng Li, Lin Yun Zhu, Si Wu Peng, Guo Qiang Chen, Pamela Moore Martin, Vadivel Ganapathy, Chi Ju Wei

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Objectives: Chronic administration of nicotinic acid (NA), a potent antilipidemic compound, aggravates glycemic control in diabetic patients. It is not known if NA has direct effects on islet β cells. Methods: Real-time reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunofluorescence techniques were used to examine the expression of NA receptor PUMA-G, a member of the G protein-coupled receptor (G-PCR) family, in murine islet β cells. Calcium transient was measured using confocal microscopy, whereas the intracellular cyclic adenosine monophosphate and glucose-stimulated insulin secretion (GSIS) from isolated islets were determined by the enzyme-linked immunosorbent assay. Results: High levels of PUMA-G transcripts and protein were detected in all β cells, and about 40% of α cells. PUMA-G transcripts increased more than 3-fold in islets incubated with interferon γ. Cyclic adenosine monophosphate accumulation, induced by IBMX/forskolin, was inhibited by NA; however, the inhibition was completely abolished by pretreatment of the culture with pertussis toxin. No calcium transient was detected in islet cells in the presence of NA. Static incubation of islets with NA led to an approximately 30% reduction of GSIS. Conclusions: The results indicated that PUMA-G stimulation by NA in islet β cells inhibited GSIS likely via activation of the Gi signaling pathway.

Original languageEnglish (US)
Pages (from-to)615-621
Number of pages7
JournalPancreas
Volume40
Issue number4
DOIs
StatePublished - May 1 2011

Keywords

  • Nicotinic acid
  • PUMA-G
  • cAMP
  • diabetes
  • insulin secretion

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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    Li, H. M., Zhang, M., Xu, S. T., Li, D. Z., Zhu, L. Y., Peng, S. W., Chen, G. Q., Martin, P. M., Ganapathy, V., & Wei, C. J. (2011). Nicotinic acid inhibits glucose-stimulated insulin secretion via the G protein-coupled receptor PUMA-G in murine islet β cells. Pancreas, 40(4), 615-621. https://doi.org/10.1097/MPA.0b013e31820b4b23