Nitric oxide delays oocyte aging

Anuradha P. Goud, Pravin T. Goud, Michael Peter Diamond, Husam M. Abu-Soud

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Nitric oxide (NO) is a ubiquitous signaling molecule that plays a crucial role in oocyte maturation and embryo development. However, its role in oocyte aging is unclear. To examine how NO affects oocyte aging, we retrieved young and relatively old mouse oocytes and exposed them to increasing concentrations of NO donor S-nitroso acetyl penicillamine (SNAP). Aging related phenomena of ooplasmic microtubule dynamics (OMD), cortical granule (CG) exocytosis, zona pellucida (ZP) hardening, and spindle/ chromatin integrity were studied at each SNAP concentration using fluorescence immunocytochemistry and confocal microscopy and compared with respective unexposed controls. Exposure of both young and old oocytes to NO resulted in a significant diminution in OMD and ZP dissolution time, whereas spontaneous CG loss decreased in old NO exposed oocytes compared to controls (P < 0.001 for all). Furthermore, NO exposure decreased the rate of spindle abnormalities in oocytes compared to unexposed controls. Interestingly, in old oocytes, the positive influence of NO was attenuated beyond 0.23 μM/min and disappeared at 0.46 μM/min NO. Overall, a significant dose-response relationship was noted between NO exposure and markers of aging with between 50 and 100 μM SNAP (0.11-0.23 μM/min NO, P < 0.0001). Collectively, our results demonstrate for the first time that exposure to NO delays oocyte aging and improves the integrity of the microtubular spindle apparatus in young and old oocytes.

Original languageEnglish (US)
Pages (from-to)11361-11368
Number of pages8
JournalBiochemistry
Volume44
Issue number34
DOIs
StatePublished - Aug 30 2005
Externally publishedYes

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Oocytes
Nitric Oxide
Aging of materials
Penicillamine
Zona Pellucida
Microtubules
Spindle Apparatus
Nitric Oxide Donors
Confocal microscopy
Exocytosis
Fluorescence Microscopy
Confocal Microscopy
Chromatin
Embryonic Development
Hardening
Dissolution
Fluorescence
Immunohistochemistry
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Goud, A. P., Goud, P. T., Diamond, M. P., & Abu-Soud, H. M. (2005). Nitric oxide delays oocyte aging. Biochemistry, 44(34), 11361-11368. https://doi.org/10.1021/bi050711f

Nitric oxide delays oocyte aging. / Goud, Anuradha P.; Goud, Pravin T.; Diamond, Michael Peter; Abu-Soud, Husam M.

In: Biochemistry, Vol. 44, No. 34, 30.08.2005, p. 11361-11368.

Research output: Contribution to journalArticle

Goud, AP, Goud, PT, Diamond, MP & Abu-Soud, HM 2005, 'Nitric oxide delays oocyte aging', Biochemistry, vol. 44, no. 34, pp. 11361-11368. https://doi.org/10.1021/bi050711f
Goud AP, Goud PT, Diamond MP, Abu-Soud HM. Nitric oxide delays oocyte aging. Biochemistry. 2005 Aug 30;44(34):11361-11368. https://doi.org/10.1021/bi050711f
Goud, Anuradha P. ; Goud, Pravin T. ; Diamond, Michael Peter ; Abu-Soud, Husam M. / Nitric oxide delays oocyte aging. In: Biochemistry. 2005 ; Vol. 44, No. 34. pp. 11361-11368.
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