Nitric oxide reduces sickle hemoglobin polymerization: Potential role of nitric oxide-induced charge alteration in depolymerization

Tohru Ikuta, Hemant S. Thatte, Jay X. Tang, Ishita Mukerji, Kelly Knee, Kenneth R. Bridges, Sabina Wang, Pedro Montero-Huerta, Ratan Mani Joshi, C. Alvin Head

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We previously demonstrated that inhaling nitric oxide (NO) increases the oxygen affinity of sickle red blood cells (RBCs) in patients with sickle cell disease (SCD). Our recent studies found that NO lowered the P50 values of sickle hemoglobin (HbS) hemolysates but did not increase methemoglobin (metHb) levels, supporting the role of NO, but not metHb, in the oxygen affinity of HbS. Here we examine the mechanism by which NO increases HbS oxygen affinity. Because anti-sickling agents increase sickle RBC oxygen affinity, we first determined whether NO exhibits anti-sickling properties. The viscosity of HbS hemolysates, measured by falling ball assays, increased upon deoxygenation; NO treatment reduced the increment. Multiphoton microscopic analyses showed smaller HbS polymers in deoxygenated sickle RBCs and HbS hemolysates exposed to NO. These results suggest that NO inhibits HbS polymer formation and has anti-sickling properties. Furthermore, we found that HbS treated with NO exhibits an isoelectric point similar to that of HbA, suggesting that NO alters the electric charge of HbS. NO-HbS adducts had the same elution time as HbA upon high performance liquid chromatography analysis. This study demonstrates that NO may disrupt HbS polymers by abolishing the excess positive charge of HbS, resulting in increased oxygen affinity.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume510
Issue number1
DOIs
StatePublished - Jun 1 2011

Fingerprint

Sickle Hemoglobin
Depolymerization
Polymerization
Nitric Oxide
Oxygen
Methemoglobin
Polymers
Blood
Erythrocytes
Accidental Falls
Electric charge
Isoelectric Point
High performance liquid chromatography
Sickle Cell Anemia
Viscosity
Inhalation
Assays

Keywords

  • Anti-sickling
  • Nitric oxide
  • Oxygen affinity
  • Polymer formation
  • Sickle cell disease

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Nitric oxide reduces sickle hemoglobin polymerization : Potential role of nitric oxide-induced charge alteration in depolymerization. / Ikuta, Tohru; Thatte, Hemant S.; Tang, Jay X.; Mukerji, Ishita; Knee, Kelly; Bridges, Kenneth R.; Wang, Sabina; Montero-Huerta, Pedro; Joshi, Ratan Mani; Head, C. Alvin.

In: Archives of Biochemistry and Biophysics, Vol. 510, No. 1, 01.06.2011, p. 53-61.

Research output: Contribution to journalArticle

Ikuta, T, Thatte, HS, Tang, JX, Mukerji, I, Knee, K, Bridges, KR, Wang, S, Montero-Huerta, P, Joshi, RM & Head, CA 2011, 'Nitric oxide reduces sickle hemoglobin polymerization: Potential role of nitric oxide-induced charge alteration in depolymerization', Archives of Biochemistry and Biophysics, vol. 510, no. 1, pp. 53-61. https://doi.org/10.1016/j.abb.2011.03.013
Ikuta, Tohru ; Thatte, Hemant S. ; Tang, Jay X. ; Mukerji, Ishita ; Knee, Kelly ; Bridges, Kenneth R. ; Wang, Sabina ; Montero-Huerta, Pedro ; Joshi, Ratan Mani ; Head, C. Alvin. / Nitric oxide reduces sickle hemoglobin polymerization : Potential role of nitric oxide-induced charge alteration in depolymerization. In: Archives of Biochemistry and Biophysics. 2011 ; Vol. 510, No. 1. pp. 53-61.
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