Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin

Cleber E. Teixeira, Juliano F. De Oliveira, Juliana S. Baracat, Fernanda B.M. Priviero, Cristina E. Okuyama, Nelson Rodrigues Netto, Adriano Fregonesi, Edson Antunes, Gilberto De Nucci

Research output: Contribution to journalArticle

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Abstract

Objectives. To investigate the effects of a purified scorpion toxin (Ts3) on human corpus cavernosum (HCC) in vitro. Scorpion venoms cause a massive release of neurotransmitters that contribute to the clinical symptoms resulting from envenomation. Methods. HCC strips were mounted in organ baths containing Krebs solution. After equilibration, the tissues were precontracted with phenylephrine (10 μmol/L). The relaxations caused by Ts3 (30 nmol/L) were compared with those induced by electrical field stimulation (1 to 20 Hz) and nitric oxide (NO, 1 to 100 μmol/L). Results. The addition of Ts3 evoked long-lasting relaxations of precontracted HCC strips, and exogenously applied NO and electrical field stimulation caused short-lived responses. The NO synthesis inhibitor Nω-nitro-L-arginine methyl ester (L-NAME; 100 μmol/L) reduced by 87% ± 2% the Ts3-induced relaxations; this inhibition was reversed by pretreating the tissues with L-arginine (1 mmol/L). The relaxant responses mediated by Ts3 were blocked to a similar degree by the soluble guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3,-alquinoxalin-1- one] (10 μmol/L). In contrast, the addition of the phosphodiesterase type 5 inhibitor sildenafil (0.1 μmol/L) significantly enhanced Ts3-evoked relaxations by 78% ± 4%. The sodium channel blocker tetrodotoxin (1 μmol/L) completely blocked the relaxant responses elicited by both Ts3 and electrical field stimulation, without significantly affecting those elicited by NO. Conclusions. The results indicate that Ts3 relaxes the HCC through the release of NO from nitrergic nerves. The elucidation of this mechanism is useful for the development of new therapeutic strategies to treat priapism after scorpion envenomation or to modulate sodium channel activity in the case of penile dysfunction.

Original languageEnglish (US)
Pages (from-to)184-189
Number of pages6
JournalUrology
Volume63
Issue number1
DOIs
StatePublished - Jan 2004

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Scorpions
Nitric Oxide
Electric Stimulation
Nitrergic Neurons
Sodium Channel Blockers
Scorpion Venoms
Priapism
Phosphodiesterase 5 Inhibitors
Sodium Channels
NG-Nitroarginine Methyl Ester
Tetrodotoxin
Phenylephrine
Baths
Neurotransmitter Agents
Arginine
Therapeutics

ASJC Scopus subject areas

  • Urology

Cite this

Teixeira, C. E., De Oliveira, J. F., Baracat, J. S., Priviero, F. B. M., Okuyama, C. E., Rodrigues Netto, N., ... De Nucci, G. (2004). Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin. Urology, 63(1), 184-189. https://doi.org/10.1016/S0090-4295(03)00785-4

Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin. / Teixeira, Cleber E.; De Oliveira, Juliano F.; Baracat, Juliana S.; Priviero, Fernanda B.M.; Okuyama, Cristina E.; Rodrigues Netto, Nelson; Fregonesi, Adriano; Antunes, Edson; De Nucci, Gilberto.

In: Urology, Vol. 63, No. 1, 01.2004, p. 184-189.

Research output: Contribution to journalArticle

Teixeira, CE, De Oliveira, JF, Baracat, JS, Priviero, FBM, Okuyama, CE, Rodrigues Netto, N, Fregonesi, A, Antunes, E & De Nucci, G 2004, 'Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin', Urology, vol. 63, no. 1, pp. 184-189. https://doi.org/10.1016/S0090-4295(03)00785-4
Teixeira CE, De Oliveira JF, Baracat JS, Priviero FBM, Okuyama CE, Rodrigues Netto N et al. Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin. Urology. 2004 Jan;63(1):184-189. https://doi.org/10.1016/S0090-4295(03)00785-4
Teixeira, Cleber E. ; De Oliveira, Juliano F. ; Baracat, Juliana S. ; Priviero, Fernanda B.M. ; Okuyama, Cristina E. ; Rodrigues Netto, Nelson ; Fregonesi, Adriano ; Antunes, Edson ; De Nucci, Gilberto. / Nitric oxide release from human corpus cavernosum induced by a purified scorpion toxin. In: Urology. 2004 ; Vol. 63, No. 1. pp. 184-189.
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abstract = "Objectives. To investigate the effects of a purified scorpion toxin (Ts3) on human corpus cavernosum (HCC) in vitro. Scorpion venoms cause a massive release of neurotransmitters that contribute to the clinical symptoms resulting from envenomation. Methods. HCC strips were mounted in organ baths containing Krebs solution. After equilibration, the tissues were precontracted with phenylephrine (10 μmol/L). The relaxations caused by Ts3 (30 nmol/L) were compared with those induced by electrical field stimulation (1 to 20 Hz) and nitric oxide (NO, 1 to 100 μmol/L). Results. The addition of Ts3 evoked long-lasting relaxations of precontracted HCC strips, and exogenously applied NO and electrical field stimulation caused short-lived responses. The NO synthesis inhibitor Nω-nitro-L-arginine methyl ester (L-NAME; 100 μmol/L) reduced by 87{\%} ± 2{\%} the Ts3-induced relaxations; this inhibition was reversed by pretreating the tissues with L-arginine (1 mmol/L). The relaxant responses mediated by Ts3 were blocked to a similar degree by the soluble guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3,-alquinoxalin-1- one] (10 μmol/L). In contrast, the addition of the phosphodiesterase type 5 inhibitor sildenafil (0.1 μmol/L) significantly enhanced Ts3-evoked relaxations by 78{\%} ± 4{\%}. The sodium channel blocker tetrodotoxin (1 μmol/L) completely blocked the relaxant responses elicited by both Ts3 and electrical field stimulation, without significantly affecting those elicited by NO. Conclusions. The results indicate that Ts3 relaxes the HCC through the release of NO from nitrergic nerves. The elucidation of this mechanism is useful for the development of new therapeutic strategies to treat priapism after scorpion envenomation or to modulate sodium channel activity in the case of penile dysfunction.",
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AU - Teixeira, Cleber E.

AU - De Oliveira, Juliano F.

AU - Baracat, Juliana S.

AU - Priviero, Fernanda B.M.

AU - Okuyama, Cristina E.

AU - Rodrigues Netto, Nelson

AU - Fregonesi, Adriano

AU - Antunes, Edson

AU - De Nucci, Gilberto

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N2 - Objectives. To investigate the effects of a purified scorpion toxin (Ts3) on human corpus cavernosum (HCC) in vitro. Scorpion venoms cause a massive release of neurotransmitters that contribute to the clinical symptoms resulting from envenomation. Methods. HCC strips were mounted in organ baths containing Krebs solution. After equilibration, the tissues were precontracted with phenylephrine (10 μmol/L). The relaxations caused by Ts3 (30 nmol/L) were compared with those induced by electrical field stimulation (1 to 20 Hz) and nitric oxide (NO, 1 to 100 μmol/L). Results. The addition of Ts3 evoked long-lasting relaxations of precontracted HCC strips, and exogenously applied NO and electrical field stimulation caused short-lived responses. The NO synthesis inhibitor Nω-nitro-L-arginine methyl ester (L-NAME; 100 μmol/L) reduced by 87% ± 2% the Ts3-induced relaxations; this inhibition was reversed by pretreating the tissues with L-arginine (1 mmol/L). The relaxant responses mediated by Ts3 were blocked to a similar degree by the soluble guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3,-alquinoxalin-1- one] (10 μmol/L). In contrast, the addition of the phosphodiesterase type 5 inhibitor sildenafil (0.1 μmol/L) significantly enhanced Ts3-evoked relaxations by 78% ± 4%. The sodium channel blocker tetrodotoxin (1 μmol/L) completely blocked the relaxant responses elicited by both Ts3 and electrical field stimulation, without significantly affecting those elicited by NO. Conclusions. The results indicate that Ts3 relaxes the HCC through the release of NO from nitrergic nerves. The elucidation of this mechanism is useful for the development of new therapeutic strategies to treat priapism after scorpion envenomation or to modulate sodium channel activity in the case of penile dysfunction.

AB - Objectives. To investigate the effects of a purified scorpion toxin (Ts3) on human corpus cavernosum (HCC) in vitro. Scorpion venoms cause a massive release of neurotransmitters that contribute to the clinical symptoms resulting from envenomation. Methods. HCC strips were mounted in organ baths containing Krebs solution. After equilibration, the tissues were precontracted with phenylephrine (10 μmol/L). The relaxations caused by Ts3 (30 nmol/L) were compared with those induced by electrical field stimulation (1 to 20 Hz) and nitric oxide (NO, 1 to 100 μmol/L). Results. The addition of Ts3 evoked long-lasting relaxations of precontracted HCC strips, and exogenously applied NO and electrical field stimulation caused short-lived responses. The NO synthesis inhibitor Nω-nitro-L-arginine methyl ester (L-NAME; 100 μmol/L) reduced by 87% ± 2% the Ts3-induced relaxations; this inhibition was reversed by pretreating the tissues with L-arginine (1 mmol/L). The relaxant responses mediated by Ts3 were blocked to a similar degree by the soluble guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3,-alquinoxalin-1- one] (10 μmol/L). In contrast, the addition of the phosphodiesterase type 5 inhibitor sildenafil (0.1 μmol/L) significantly enhanced Ts3-evoked relaxations by 78% ± 4%. The sodium channel blocker tetrodotoxin (1 μmol/L) completely blocked the relaxant responses elicited by both Ts3 and electrical field stimulation, without significantly affecting those elicited by NO. Conclusions. The results indicate that Ts3 relaxes the HCC through the release of NO from nitrergic nerves. The elucidation of this mechanism is useful for the development of new therapeutic strategies to treat priapism after scorpion envenomation or to modulate sodium channel activity in the case of penile dysfunction.

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