NMDA- and endothelin-1-induced increases in blood-brain barrier permeability quantitated with Lucifer yellow

R. Daniel Miller, Nicholas T. Monsul, John R. Vender, John C. Lehmann

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

At 48 h following intrastriatal injection of N-methyl-D-aspartate (NMDA; 100 nmol/μl) or endothelin-l (ET-1; 143 pmol/μl), significant increases in brain penetration of the highly polar, fluorescent tracer Lucifer yellow were observed. The competitive NMDA receptor antagonist selfotel (CGS-19755; 30 nmol/μl, i.c.) significantly reduced the NMDA-induced increases in blood-brain barrier permeability, but not those induced by ET-1. These results suggest that NMDA receptors can mediate increases in blood-brain barrier permeability but do not primarily mediate increases in blood-brain barrier permeability caused by ET-1. This is the first study to our knowledge investigating the relationship between excitotoxicity and disruption of the blood-brain barrier, a major pathophysiological event in stroke and traumatic brain injury.

Original languageEnglish (US)
Pages (from-to)37-40
Number of pages4
JournalJournal of the Neurological Sciences
Volume136
Issue number1-2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • Blood-brain barrier
  • Cerebrovasculature
  • Endothelin-1 (ET-1)
  • Excitatory amino acid
  • Lucifer yellow
  • N-Methyl-D-aspartate
  • Selfotel

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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