NMDA receptor/L-VGCC-dependent expression and AMPA/KA receptor-dependent activation of c-Jun induced by cerebral ischemia in rat hippocampus

Quan Guang Zhang, Yong Ling Xu, Hong Chun Li, Dong Han, Guang Yi Zhang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Over-activation of ionotropic glutamate receptors can cause an excessive influx of calcium ions into neurons, which subsequently triggers the degeneration and death of cells in a process known as excitotoxicity. Here, we examined the effects of modulating ionotropic glutamate receptors and L-type voltage-gated calcium channels (L-VGCC) on the expression and activation of c-Jun in hippocampus of SD rats after transient global ischemia. The total protein of c-Jun was altered by ischemia-reperfusion and reached its high levels at 3-6 h of reperfusion. However, the increased expression was prevented by pretreatment of ketamine (a non-competitive N-methyl-d-aspartate (NMDA) receptors antagonist) or nifedipine (a blocker of L-VGCC), but not by 6,7-dinitroquinoxaline-2,3(1H,4H)-dione (DNQX), an AMPA/KA receptor antagonist. On the other hand, c-Jun phosphorylation was significantly increased 3 h after reperfusion, which was inhibited by DNQX, but not ketamine or nifedipine. AP-1 binding activity reactions were also performed by electrophoretic mobility shift assay (EMSA), which detected similar results as those in Western blotting. Our results clearly showed that c-Jun expression is NMDA receptor/L-VGCC-dependent and c-Jun activation is AMPA/KA receptor-dependent, which expands our knowledge of the JNK-c-Jun signaling pathway in ischemic brain damage.

Original languageEnglish (US)
Pages (from-to)268-273
Number of pages6
JournalNeuroscience Letters
Volume398
Issue number3
DOIs
StatePublished - May 8 2006

Fingerprint

L-Type Calcium Channels
AMPA Receptors
Brain Ischemia
Reperfusion
Ionotropic Glutamate Receptors
Hippocampus
Ketamine
Calcium Channels
Nifedipine
Ischemia
Proto-Oncogene Proteins c-jun
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Cell Death
Western Blotting
Phosphorylation
Ions
Calcium
Neurons
Brain

Keywords

  • AMPA/KA receptor
  • Antagonist
  • Cerebral ischemia
  • L-Type voltage-gated Ca channel
  • NMDA receptor
  • Rat
  • c-Jun

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

NMDA receptor/L-VGCC-dependent expression and AMPA/KA receptor-dependent activation of c-Jun induced by cerebral ischemia in rat hippocampus. / Zhang, Quan Guang; Xu, Yong Ling; Li, Hong Chun; Han, Dong; Zhang, Guang Yi.

In: Neuroscience Letters, Vol. 398, No. 3, 08.05.2006, p. 268-273.

Research output: Contribution to journalArticle

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