Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5

Shumin Zhou, Wei Xiao, Qingwen Wan, Changhua Yi, Feifei Xiao, Yingle Liu, Yipeng Qi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Nogo-B is a known regulator of neural functions and plays an important role in cell adhesion and migration. To our knowledge, the molecular mechanism behind its regulation of cell motility is still unknown. Here, we identified Fibulin-5, a secreted extracellular matrix protein, as a binding partner of Nogo-B. Using HeLa cells as a model, we found that Nogo-B and Fibulin-5 co-localize in the cytoplasm and plasma membrane. Furthermore, in HeLa cells that overexpress Nogo-B, cell migration and invasion was promoted by the elevated secretion of Fibulin-5. Thus, identification of the Nogo-B binding protein Fibulin-5 may contribute to uncover the pathway in which Nogo-B regulates tumor cell movement.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume398
Issue number2
DOIs
StatePublished - Jul 1 2010

Keywords

  • Cell motility
  • Fibulin-5
  • Nogo-B
  • Secretion
  • Tumor metastasis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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