Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5

Shumin Zhou; Wei Xiao; Qingwen Wan; Changhua Yi; Feifei Xiao; Yingle Liu; Yipeng Qi

doi:10.1016/j.bbrc.2010.06.068

Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5

Shumin Zhou, Wei Xiao, Qingwen Wan, Changhua Yi, Feifei Xiao, Yingle Liu, Yipeng Qi

Neurology

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

Nogo-B is a known regulator of neural functions and plays an important role in cell adhesion and migration. To our knowledge, the molecular mechanism behind its regulation of cell motility is still unknown. Here, we identified Fibulin-5, a secreted extracellular matrix protein, as a binding partner of Nogo-B. Using HeLa cells as a model, we found that Nogo-B and Fibulin-5 co-localize in the cytoplasm and plasma membrane. Furthermore, in HeLa cells that overexpress Nogo-B, cell migration and invasion was promoted by the elevated secretion of Fibulin-5. Thus, identification of the Nogo-B binding protein Fibulin-5 may contribute to uncover the pathway in which Nogo-B regulates tumor cell movement.

Original language	English (US)
Pages (from-to)	247-253
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	398
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.06.068
State	Published - Jul 1 2010

Keywords

Cell motility
Fibulin-5
Nogo-B
Secretion
Tumor metastasis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Zhou, S., Xiao, W., Wan, Q., Yi, C., Xiao, F., Liu, Y., & Qi, Y. (2010). Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5. Biochemical and Biophysical Research Communications, 398(2), 247-253. https://doi.org/10.1016/j.bbrc.2010.06.068

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AU - Liu, Yingle

AU - Qi, Yipeng

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