Abstract
Nogo-B is a known regulator of neural functions and plays an important role in cell adhesion and migration. To our knowledge, the molecular mechanism behind its regulation of cell motility is still unknown. Here, we identified Fibulin-5, a secreted extracellular matrix protein, as a binding partner of Nogo-B. Using HeLa cells as a model, we found that Nogo-B and Fibulin-5 co-localize in the cytoplasm and plasma membrane. Furthermore, in HeLa cells that overexpress Nogo-B, cell migration and invasion was promoted by the elevated secretion of Fibulin-5. Thus, identification of the Nogo-B binding protein Fibulin-5 may contribute to uncover the pathway in which Nogo-B regulates tumor cell movement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 247-253 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 398 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 1 2010 |
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Keywords
- Cell motility
- Fibulin-5
- Nogo-B
- Secretion
- Tumor metastasis
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5. / Zhou, Shumin; Xiao, Wei; Wan, Qingwen; Yi, Changhua; Xiao, Feifei; Liu, Yingle; Qi, Yipeng.
In: Biochemical and Biophysical Research Communications, Vol. 398, No. 2, 01.07.2010, p. 247-253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nogo-B mediates HeLa cell adhesion and motility through binding of Fibulin-5
AU - Zhou, Shumin
AU - Xiao, Wei
AU - Wan, Qingwen
AU - Yi, Changhua
AU - Xiao, Feifei
AU - Liu, Yingle
AU - Qi, Yipeng
PY - 2010/7/1
Y1 - 2010/7/1
N2 - Nogo-B is a known regulator of neural functions and plays an important role in cell adhesion and migration. To our knowledge, the molecular mechanism behind its regulation of cell motility is still unknown. Here, we identified Fibulin-5, a secreted extracellular matrix protein, as a binding partner of Nogo-B. Using HeLa cells as a model, we found that Nogo-B and Fibulin-5 co-localize in the cytoplasm and plasma membrane. Furthermore, in HeLa cells that overexpress Nogo-B, cell migration and invasion was promoted by the elevated secretion of Fibulin-5. Thus, identification of the Nogo-B binding protein Fibulin-5 may contribute to uncover the pathway in which Nogo-B regulates tumor cell movement.
AB - Nogo-B is a known regulator of neural functions and plays an important role in cell adhesion and migration. To our knowledge, the molecular mechanism behind its regulation of cell motility is still unknown. Here, we identified Fibulin-5, a secreted extracellular matrix protein, as a binding partner of Nogo-B. Using HeLa cells as a model, we found that Nogo-B and Fibulin-5 co-localize in the cytoplasm and plasma membrane. Furthermore, in HeLa cells that overexpress Nogo-B, cell migration and invasion was promoted by the elevated secretion of Fibulin-5. Thus, identification of the Nogo-B binding protein Fibulin-5 may contribute to uncover the pathway in which Nogo-B regulates tumor cell movement.
KW - Cell motility
KW - Fibulin-5
KW - Nogo-B
KW - Secretion
KW - Tumor metastasis
UR - http://www.scopus.com/inward/record.url?scp=77955058436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955058436&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.06.068
DO - 10.1016/j.bbrc.2010.06.068
M3 - Article
C2 - 20599731
AN - SCOPUS:77955058436
VL - 398
SP - 247
EP - 253
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -