Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter

Y. Albert Pan, Joshua R. Sanes

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We describe transgenic mice in which expression of a reporter, the yellow fluorescent protein (YFP), is locally activated in epidermal cells at sites of injury. YFP is detectable a day after injury; its levels peak within 3 d, and then decline over the subsequent week. Expression is also activated by a chemical irritant, and is suppressed by topical administration of hydrocortisone. These mice permit non-invasive time-lapse monitoring of responses to injury in vivo. They can be used to detect epidermal activation and to test agents that may provoke or attenuate epidermal responses.

Original languageEnglish (US)
Pages (from-to)888-891
Number of pages4
JournalJournal of Investigative Dermatology
Volume123
Issue number5
DOIs
StatePublished - Nov 2004

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Keywords

  • Epidermis
  • Fluorescent
  • In vivo
  • Keratinocytes
  • Skin trauma
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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