Abstract
We describe transgenic mice in which expression of a reporter, the yellow fluorescent protein (YFP), is locally activated in epidermal cells at sites of injury. YFP is detectable a day after injury; its levels peak within 3 d, and then decline over the subsequent week. Expression is also activated by a chemical irritant, and is suppressed by topical administration of hydrocortisone. These mice permit non-invasive time-lapse monitoring of responses to injury in vivo. They can be used to detect epidermal activation and to test agents that may provoke or attenuate epidermal responses.
Original language | English (US) |
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Pages (from-to) | 888-891 |
Number of pages | 4 |
Journal | Journal of Investigative Dermatology |
Volume | 123 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2004 |
Externally published | Yes |
Keywords
- Epidermis
- Fluorescent
- In vivo
- Keratinocytes
- Skin trauma
- Transgenic mice
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology