Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter

Yu Chin Albert Pan, Joshua R. Sanes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We describe transgenic mice in which expression of a reporter, the yellow fluorescent protein (YFP), is locally activated in epidermal cells at sites of injury. YFP is detectable a day after injury; its levels peak within 3 d, and then decline over the subsequent week. Expression is also activated by a chemical irritant, and is suppressed by topical administration of hydrocortisone. These mice permit non-invasive time-lapse monitoring of responses to injury in vivo. They can be used to detect epidermal activation and to test agents that may provoke or attenuate epidermal responses.

Original languageEnglish (US)
Pages (from-to)888-891
Number of pages4
JournalJournal of Investigative Dermatology
Volume123
Issue number5
DOIs
StatePublished - Jan 1 2004

Fingerprint

Visualization
Irritants
Wounds and Injuries
Hydrocortisone
Topical Administration
Proteins
Chemical activation
Transgenic Mice
Monitoring

Keywords

  • Epidermis
  • Fluorescent
  • In vivo
  • Keratinocytes
  • Skin trauma
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Non-invasive visualization of epidermal responses to injury using a fluorescent transgenic reporter. / Pan, Yu Chin Albert; Sanes, Joshua R.

In: Journal of Investigative Dermatology, Vol. 123, No. 5, 01.01.2004, p. 888-891.

Research output: Contribution to journalArticle

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