Nonclassical binding of formylated peptide in crystal structure of the MHC class lb molecule H2-M3

Chyung Ru Wang, A. Raúl Castaño, Per A. Peterson, Clive A. Slaughter, Kirsten Fischer Lindahl, Johann Deisenhofer

Research output: Contribution to journalArticle

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Abstract

H2-M3 is a class Ib MHC molecule of the mouse with a 104-fold preference for binding N-fonmylated peptides. To elucidate the basis of this unusual specificity, we expressed and crystallized a soluble form of M3 with a fonnylated nonamer peptide, fMYFINILTL, and determined the structure by X-ray crystallography. M3, refined at 2.1 Å resolution, resembles class la MHC molecules in its overall structure, but differs in the peptide-binding groove. The A pocket, which usually accommodates the free N-terminus of a bound peptide, is closed, and the peptide Is shifted one residue, such that the P1 side chain is lodged in the B pocket. The formyl group Is coordinated by His-9 and a bound water on the floor of the groove.

Original languageEnglish (US)
Pages (from-to)655-664
Number of pages10
JournalCell
Volume82
Issue number4
DOIs
StatePublished - Aug 25 1995

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Crystal structure
Peptides
Molecules
X ray crystallography
X Ray Crystallography
Water

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wang, C. R., Castaño, A. R., Peterson, P. A., Slaughter, C. A., Fischer Lindahl, K., & Deisenhofer, J. (1995). Nonclassical binding of formylated peptide in crystal structure of the MHC class lb molecule H2-M3. Cell, 82(4), 655-664. https://doi.org/10.1016/0092-8674(95)90037-3

Nonclassical binding of formylated peptide in crystal structure of the MHC class lb molecule H2-M3. / Wang, Chyung Ru; Castaño, A. Raúl; Peterson, Per A.; Slaughter, Clive A.; Fischer Lindahl, Kirsten; Deisenhofer, Johann.

In: Cell, Vol. 82, No. 4, 25.08.1995, p. 655-664.

Research output: Contribution to journalArticle

Wang, CR, Castaño, AR, Peterson, PA, Slaughter, CA, Fischer Lindahl, K & Deisenhofer, J 1995, 'Nonclassical binding of formylated peptide in crystal structure of the MHC class lb molecule H2-M3', Cell, vol. 82, no. 4, pp. 655-664. https://doi.org/10.1016/0092-8674(95)90037-3
Wang, Chyung Ru ; Castaño, A. Raúl ; Peterson, Per A. ; Slaughter, Clive A. ; Fischer Lindahl, Kirsten ; Deisenhofer, Johann. / Nonclassical binding of formylated peptide in crystal structure of the MHC class lb molecule H2-M3. In: Cell. 1995 ; Vol. 82, No. 4. pp. 655-664.
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