Nonshivering thermogenesis in adult and aged C57BL/6J mice housed at 22°C and at 29°C

Mark I. Talan, Sergei A Kirov, Nadejda A. Kosheleva

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Twelve and 28-month-old C57BL/6J male mice were housed either at room temperature of 22°C or at thermoneutrality (29°C) during the two months prior to experiments. Acute experiments were conducted under anesthesia, myorelaxation, and artificial ventilation. We recorded efferent electrical impulse activity in one of the sympathetic nerves innervating the interscapular brown adipose tissue in response to acute cold stimulation, when body temperature was lowered 7.5°C below control level. In separate experiments we measured O2 consumption and CO2 production and calculated the nonshivering thermogenesis. We also measured the concentration of uncoupling protein in interscapular brown adipose tissue before and after three-hour cold stress. In aged mice, both sympathetic nervous activity and nonshivering thermogenesis were lower in animals housed at thermoneutrality (29°C) than in mice housed at 22°. Among mice maintained at 22°C, but not at thermoneutrality, aged animals had greater nonshivering thermogenesis and greater cold induced concentration of uncoupling protein in the brown adipose tissue than adults. Sympathetic nervous outflow to brown adipose tissue was always greater in aged mice, regardless of the temperature of acclimation. We concluded that aged mice, housed at 22°C, showed the changes in nonshivering thermogenesis associated with cold acclimation. However, an increased sympathetic outflow to brown adipose tissue in aged animals reflects an age- related elevation of the tone and responsiveness of the sympathetic nervous system.

Original languageEnglish (US)
Pages (from-to)687-698
Number of pages12
JournalExperimental Gerontology
Volume31
Issue number6
DOIs
StatePublished - Nov 1 1996
Externally publishedYes

Fingerprint

Thermogenesis
Inbred C57BL Mouse
Brown Adipose Tissue
Tissue
Animals
Acclimatization
Experiments
Level control
Neurology
Temperature
Sympathetic Nervous System
Body Temperature
Proteins
Anesthesia

Keywords

  • body temperature
  • brown adipose tissue
  • cold tolerance
  • heat production
  • rodents
  • sympathetic nervous activity
  • thermogenin
  • thermoregulation
  • uncoupling protein

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Nonshivering thermogenesis in adult and aged C57BL/6J mice housed at 22°C and at 29°C. / Talan, Mark I.; Kirov, Sergei A; Kosheleva, Nadejda A.

In: Experimental Gerontology, Vol. 31, No. 6, 01.11.1996, p. 687-698.

Research output: Contribution to journalArticle

@article{8c0247b2d1fe44ceb9fe9f20a2b73347,
title = "Nonshivering thermogenesis in adult and aged C57BL/6J mice housed at 22°C and at 29°C",
abstract = "Twelve and 28-month-old C57BL/6J male mice were housed either at room temperature of 22°C or at thermoneutrality (29°C) during the two months prior to experiments. Acute experiments were conducted under anesthesia, myorelaxation, and artificial ventilation. We recorded efferent electrical impulse activity in one of the sympathetic nerves innervating the interscapular brown adipose tissue in response to acute cold stimulation, when body temperature was lowered 7.5°C below control level. In separate experiments we measured O2 consumption and CO2 production and calculated the nonshivering thermogenesis. We also measured the concentration of uncoupling protein in interscapular brown adipose tissue before and after three-hour cold stress. In aged mice, both sympathetic nervous activity and nonshivering thermogenesis were lower in animals housed at thermoneutrality (29°C) than in mice housed at 22°. Among mice maintained at 22°C, but not at thermoneutrality, aged animals had greater nonshivering thermogenesis and greater cold induced concentration of uncoupling protein in the brown adipose tissue than adults. Sympathetic nervous outflow to brown adipose tissue was always greater in aged mice, regardless of the temperature of acclimation. We concluded that aged mice, housed at 22°C, showed the changes in nonshivering thermogenesis associated with cold acclimation. However, an increased sympathetic outflow to brown adipose tissue in aged animals reflects an age- related elevation of the tone and responsiveness of the sympathetic nervous system.",
keywords = "body temperature, brown adipose tissue, cold tolerance, heat production, rodents, sympathetic nervous activity, thermogenin, thermoregulation, uncoupling protein",
author = "Talan, {Mark I.} and Kirov, {Sergei A} and Kosheleva, {Nadejda A.}",
year = "1996",
month = "11",
day = "1",
doi = "10.1016/S0531-5565(96)00095-2",
language = "English (US)",
volume = "31",
pages = "687--698",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Nonshivering thermogenesis in adult and aged C57BL/6J mice housed at 22°C and at 29°C

AU - Talan, Mark I.

AU - Kirov, Sergei A

AU - Kosheleva, Nadejda A.

PY - 1996/11/1

Y1 - 1996/11/1

N2 - Twelve and 28-month-old C57BL/6J male mice were housed either at room temperature of 22°C or at thermoneutrality (29°C) during the two months prior to experiments. Acute experiments were conducted under anesthesia, myorelaxation, and artificial ventilation. We recorded efferent electrical impulse activity in one of the sympathetic nerves innervating the interscapular brown adipose tissue in response to acute cold stimulation, when body temperature was lowered 7.5°C below control level. In separate experiments we measured O2 consumption and CO2 production and calculated the nonshivering thermogenesis. We also measured the concentration of uncoupling protein in interscapular brown adipose tissue before and after three-hour cold stress. In aged mice, both sympathetic nervous activity and nonshivering thermogenesis were lower in animals housed at thermoneutrality (29°C) than in mice housed at 22°. Among mice maintained at 22°C, but not at thermoneutrality, aged animals had greater nonshivering thermogenesis and greater cold induced concentration of uncoupling protein in the brown adipose tissue than adults. Sympathetic nervous outflow to brown adipose tissue was always greater in aged mice, regardless of the temperature of acclimation. We concluded that aged mice, housed at 22°C, showed the changes in nonshivering thermogenesis associated with cold acclimation. However, an increased sympathetic outflow to brown adipose tissue in aged animals reflects an age- related elevation of the tone and responsiveness of the sympathetic nervous system.

AB - Twelve and 28-month-old C57BL/6J male mice were housed either at room temperature of 22°C or at thermoneutrality (29°C) during the two months prior to experiments. Acute experiments were conducted under anesthesia, myorelaxation, and artificial ventilation. We recorded efferent electrical impulse activity in one of the sympathetic nerves innervating the interscapular brown adipose tissue in response to acute cold stimulation, when body temperature was lowered 7.5°C below control level. In separate experiments we measured O2 consumption and CO2 production and calculated the nonshivering thermogenesis. We also measured the concentration of uncoupling protein in interscapular brown adipose tissue before and after three-hour cold stress. In aged mice, both sympathetic nervous activity and nonshivering thermogenesis were lower in animals housed at thermoneutrality (29°C) than in mice housed at 22°. Among mice maintained at 22°C, but not at thermoneutrality, aged animals had greater nonshivering thermogenesis and greater cold induced concentration of uncoupling protein in the brown adipose tissue than adults. Sympathetic nervous outflow to brown adipose tissue was always greater in aged mice, regardless of the temperature of acclimation. We concluded that aged mice, housed at 22°C, showed the changes in nonshivering thermogenesis associated with cold acclimation. However, an increased sympathetic outflow to brown adipose tissue in aged animals reflects an age- related elevation of the tone and responsiveness of the sympathetic nervous system.

KW - body temperature

KW - brown adipose tissue

KW - cold tolerance

KW - heat production

KW - rodents

KW - sympathetic nervous activity

KW - thermogenin

KW - thermoregulation

KW - uncoupling protein

UR - http://www.scopus.com/inward/record.url?scp=0030296499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030296499&partnerID=8YFLogxK

U2 - 10.1016/S0531-5565(96)00095-2

DO - 10.1016/S0531-5565(96)00095-2

M3 - Article

C2 - 9415097

AN - SCOPUS:0030296499

VL - 31

SP - 687

EP - 698

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

IS - 6

ER -