Normal cone function requires the interphotoreceptor retinoid binding protein

Ryan O. Parker, Jie Fan, John M. Nickerson, Gregory I Liou, Rosalie K. Crouch

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

11-cis-retinal is the light-sensitive component in rod and cone photoreceptors, and its isomerization to all-trans retinal in the presence of light initiates the visual response. For photoreceptors to function normally, all-trans retinal must be converted back into 11-cis-retinal through a series of enzymatic steps known as the visual cycle. The interphotoreceptor retinoid-binding protein (IRBP) is a proposed retinoid transporter in the visual cycle, but rods in Irbp -/- mice have a normal visual cycle. While rods are primarily responsible for dim light vision, the ability of cones to function in constant light is essential to human vision and may be facilitated by cone-specific visual cycle pathways. We analyzed the cones in Irbp -/- mice to determine whether IRBP has a cone-specific visual cycle function. Cone electroretinogram (ERG) responses were reduced in Irbp -/- mice, but similar responses from Irbp -/- mice at all ages suggest that degeneration does not underlie cone dysfunction. Furthermore, cone densities and opsin levels in Irbp -/- mice were similar to C57BL/6 (wild-type) mice, and both cone opsins were properly localized to the cone outer segments. To test for retinoid deficiency in Irbp -/- mice, ERGs were analyzed before and after intraperitoneal injections of 9-cis-retinal. Treatment with 9-cis-retinal produced a significant recovery of the cone response in Irbp -/- mice and shows that retinoid deficiency underlies cone dysfunction. These data indicate that IRBP is essential to normal cone function and demonstrate that differences exist in the visual cycle of rods and cones.

Original languageEnglish (US)
Pages (from-to)4616-4621
Number of pages6
JournalJournal of Neuroscience
Volume29
Issue number14
DOIs
StatePublished - Apr 8 2009

Fingerprint

Retinoids
Cone Opsins
Retinaldehyde
Vertebrate Photoreceptor Cells
Light
Retinal Cone Photoreceptor Cells
Retinal Rod Photoreceptor Cells
Aptitude
Visual Pathways
interstitial retinol-binding protein
Intraperitoneal Injections
9-cis-retinal
Therapeutics

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Parker, R. O., Fan, J., Nickerson, J. M., Liou, G. I., & Crouch, R. K. (2009). Normal cone function requires the interphotoreceptor retinoid binding protein. Journal of Neuroscience, 29(14), 4616-4621. https://doi.org/10.1523/JNEUROSCI.0063-09.2009

Normal cone function requires the interphotoreceptor retinoid binding protein. / Parker, Ryan O.; Fan, Jie; Nickerson, John M.; Liou, Gregory I; Crouch, Rosalie K.

In: Journal of Neuroscience, Vol. 29, No. 14, 08.04.2009, p. 4616-4621.

Research output: Contribution to journalArticle

Parker, RO, Fan, J, Nickerson, JM, Liou, GI & Crouch, RK 2009, 'Normal cone function requires the interphotoreceptor retinoid binding protein', Journal of Neuroscience, vol. 29, no. 14, pp. 4616-4621. https://doi.org/10.1523/JNEUROSCI.0063-09.2009
Parker, Ryan O. ; Fan, Jie ; Nickerson, John M. ; Liou, Gregory I ; Crouch, Rosalie K. / Normal cone function requires the interphotoreceptor retinoid binding protein. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 14. pp. 4616-4621.
@article{95c95340a142463496661c0dcac2e924,
title = "Normal cone function requires the interphotoreceptor retinoid binding protein",
abstract = "11-cis-retinal is the light-sensitive component in rod and cone photoreceptors, and its isomerization to all-trans retinal in the presence of light initiates the visual response. For photoreceptors to function normally, all-trans retinal must be converted back into 11-cis-retinal through a series of enzymatic steps known as the visual cycle. The interphotoreceptor retinoid-binding protein (IRBP) is a proposed retinoid transporter in the visual cycle, but rods in Irbp -/- mice have a normal visual cycle. While rods are primarily responsible for dim light vision, the ability of cones to function in constant light is essential to human vision and may be facilitated by cone-specific visual cycle pathways. We analyzed the cones in Irbp -/- mice to determine whether IRBP has a cone-specific visual cycle function. Cone electroretinogram (ERG) responses were reduced in Irbp -/- mice, but similar responses from Irbp -/- mice at all ages suggest that degeneration does not underlie cone dysfunction. Furthermore, cone densities and opsin levels in Irbp -/- mice were similar to C57BL/6 (wild-type) mice, and both cone opsins were properly localized to the cone outer segments. To test for retinoid deficiency in Irbp -/- mice, ERGs were analyzed before and after intraperitoneal injections of 9-cis-retinal. Treatment with 9-cis-retinal produced a significant recovery of the cone response in Irbp -/- mice and shows that retinoid deficiency underlies cone dysfunction. These data indicate that IRBP is essential to normal cone function and demonstrate that differences exist in the visual cycle of rods and cones.",
author = "Parker, {Ryan O.} and Jie Fan and Nickerson, {John M.} and Liou, {Gregory I} and Crouch, {Rosalie K.}",
year = "2009",
month = "4",
day = "8",
doi = "10.1523/JNEUROSCI.0063-09.2009",
language = "English (US)",
volume = "29",
pages = "4616--4621",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "14",

}

TY - JOUR

T1 - Normal cone function requires the interphotoreceptor retinoid binding protein

AU - Parker, Ryan O.

AU - Fan, Jie

AU - Nickerson, John M.

AU - Liou, Gregory I

AU - Crouch, Rosalie K.

PY - 2009/4/8

Y1 - 2009/4/8

N2 - 11-cis-retinal is the light-sensitive component in rod and cone photoreceptors, and its isomerization to all-trans retinal in the presence of light initiates the visual response. For photoreceptors to function normally, all-trans retinal must be converted back into 11-cis-retinal through a series of enzymatic steps known as the visual cycle. The interphotoreceptor retinoid-binding protein (IRBP) is a proposed retinoid transporter in the visual cycle, but rods in Irbp -/- mice have a normal visual cycle. While rods are primarily responsible for dim light vision, the ability of cones to function in constant light is essential to human vision and may be facilitated by cone-specific visual cycle pathways. We analyzed the cones in Irbp -/- mice to determine whether IRBP has a cone-specific visual cycle function. Cone electroretinogram (ERG) responses were reduced in Irbp -/- mice, but similar responses from Irbp -/- mice at all ages suggest that degeneration does not underlie cone dysfunction. Furthermore, cone densities and opsin levels in Irbp -/- mice were similar to C57BL/6 (wild-type) mice, and both cone opsins were properly localized to the cone outer segments. To test for retinoid deficiency in Irbp -/- mice, ERGs were analyzed before and after intraperitoneal injections of 9-cis-retinal. Treatment with 9-cis-retinal produced a significant recovery of the cone response in Irbp -/- mice and shows that retinoid deficiency underlies cone dysfunction. These data indicate that IRBP is essential to normal cone function and demonstrate that differences exist in the visual cycle of rods and cones.

AB - 11-cis-retinal is the light-sensitive component in rod and cone photoreceptors, and its isomerization to all-trans retinal in the presence of light initiates the visual response. For photoreceptors to function normally, all-trans retinal must be converted back into 11-cis-retinal through a series of enzymatic steps known as the visual cycle. The interphotoreceptor retinoid-binding protein (IRBP) is a proposed retinoid transporter in the visual cycle, but rods in Irbp -/- mice have a normal visual cycle. While rods are primarily responsible for dim light vision, the ability of cones to function in constant light is essential to human vision and may be facilitated by cone-specific visual cycle pathways. We analyzed the cones in Irbp -/- mice to determine whether IRBP has a cone-specific visual cycle function. Cone electroretinogram (ERG) responses were reduced in Irbp -/- mice, but similar responses from Irbp -/- mice at all ages suggest that degeneration does not underlie cone dysfunction. Furthermore, cone densities and opsin levels in Irbp -/- mice were similar to C57BL/6 (wild-type) mice, and both cone opsins were properly localized to the cone outer segments. To test for retinoid deficiency in Irbp -/- mice, ERGs were analyzed before and after intraperitoneal injections of 9-cis-retinal. Treatment with 9-cis-retinal produced a significant recovery of the cone response in Irbp -/- mice and shows that retinoid deficiency underlies cone dysfunction. These data indicate that IRBP is essential to normal cone function and demonstrate that differences exist in the visual cycle of rods and cones.

UR - http://www.scopus.com/inward/record.url?scp=65549130215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549130215&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.0063-09.2009

DO - 10.1523/JNEUROSCI.0063-09.2009

M3 - Article

C2 - 19357286

AN - SCOPUS:65549130215

VL - 29

SP - 4616

EP - 4621

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 14

ER -