Normoxic wound fluid contains high levels of vascular endothelial growth factor

Thomas R. Howdieshell, Charlton Riegner, Vinay Gupta, Dianne Callaway, Ken Grembowicz, Sathyanarayana, Paul L McNeil

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Objective: To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. Summary Background Data: During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. Methods: A ventral hernia, surgically created in the abdominal wall of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for PO2, PCO2, pH, and growth factor concentrations. Results: Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. Conclusions: The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.

Original languageEnglish (US)
Pages (from-to)707-715
Number of pages9
JournalAnnals of Surgery
Volume228
Issue number5
DOIs
StatePublished - Nov 1 1998

Fingerprint

Vascular Endothelial Growth Factor A
Wounds and Injuries
Granulation Tissue
Subcutaneous Tissue
Transforming Growth Factor beta
Intercellular Signaling Peptides and Proteins
Ventral Hernia
Skin
Omentum
Capillary Permeability
Abdominal Wall
Silicones
Wound Healing
Permeability
Ultrasonography
Anatomy
Proteins
Neutrophils
Swine
Fibroblasts

ASJC Scopus subject areas

  • Surgery

Cite this

Howdieshell, T. R., Riegner, C., Gupta, V., Callaway, D., Grembowicz, K., Sathyanarayana, & McNeil, P. L. (1998). Normoxic wound fluid contains high levels of vascular endothelial growth factor. Annals of Surgery, 228(5), 707-715. https://doi.org/10.1097/00000658-199811000-00011

Normoxic wound fluid contains high levels of vascular endothelial growth factor. / Howdieshell, Thomas R.; Riegner, Charlton; Gupta, Vinay; Callaway, Dianne; Grembowicz, Ken; Sathyanarayana; McNeil, Paul L.

In: Annals of Surgery, Vol. 228, No. 5, 01.11.1998, p. 707-715.

Research output: Contribution to journalArticle

Howdieshell, TR, Riegner, C, Gupta, V, Callaway, D, Grembowicz, K, Sathyanarayana & McNeil, PL 1998, 'Normoxic wound fluid contains high levels of vascular endothelial growth factor', Annals of Surgery, vol. 228, no. 5, pp. 707-715. https://doi.org/10.1097/00000658-199811000-00011
Howdieshell TR, Riegner C, Gupta V, Callaway D, Grembowicz K, Sathyanarayana et al. Normoxic wound fluid contains high levels of vascular endothelial growth factor. Annals of Surgery. 1998 Nov 1;228(5):707-715. https://doi.org/10.1097/00000658-199811000-00011
Howdieshell, Thomas R. ; Riegner, Charlton ; Gupta, Vinay ; Callaway, Dianne ; Grembowicz, Ken ; Sathyanarayana ; McNeil, Paul L. / Normoxic wound fluid contains high levels of vascular endothelial growth factor. In: Annals of Surgery. 1998 ; Vol. 228, No. 5. pp. 707-715.
@article{369e8f1096b54a0a91f80e604f8273fb,
title = "Normoxic wound fluid contains high levels of vascular endothelial growth factor",
abstract = "Objective: To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. Summary Background Data: During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. Methods: A ventral hernia, surgically created in the abdominal wall of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for PO2, PCO2, pH, and growth factor concentrations. Results: Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. Conclusions: The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.",
author = "Howdieshell, {Thomas R.} and Charlton Riegner and Vinay Gupta and Dianne Callaway and Ken Grembowicz and Sathyanarayana and McNeil, {Paul L}",
year = "1998",
month = "11",
day = "1",
doi = "10.1097/00000658-199811000-00011",
language = "English (US)",
volume = "228",
pages = "707--715",
journal = "Annals of Surgery",
issn = "0003-4932",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Normoxic wound fluid contains high levels of vascular endothelial growth factor

AU - Howdieshell, Thomas R.

AU - Riegner, Charlton

AU - Gupta, Vinay

AU - Callaway, Dianne

AU - Grembowicz, Ken

AU - Sathyanarayana,

AU - McNeil, Paul L

PY - 1998/11/1

Y1 - 1998/11/1

N2 - Objective: To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. Summary Background Data: During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. Methods: A ventral hernia, surgically created in the abdominal wall of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for PO2, PCO2, pH, and growth factor concentrations. Results: Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. Conclusions: The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.

AB - Objective: To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. Summary Background Data: During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. Methods: A ventral hernia, surgically created in the abdominal wall of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for PO2, PCO2, pH, and growth factor concentrations. Results: Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. Conclusions: The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.

UR - http://www.scopus.com/inward/record.url?scp=0032470748&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032470748&partnerID=8YFLogxK

U2 - 10.1097/00000658-199811000-00011

DO - 10.1097/00000658-199811000-00011

M3 - Article

VL - 228

SP - 707

EP - 715

JO - Annals of Surgery

JF - Annals of Surgery

SN - 0003-4932

IS - 5

ER -