Novel anticoagulant activity of polybrene

Inhibition of monocytic tissue factor hypercoagulation following bacterial endotoxin induction

Arthur J. Chu, M. Rauci, Obioma Ikechukwu Nwobi, S. T. Mathews, S. Beydoun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The enhanced extrinsic coagulation in response to inflammation could contribute to disseminated intravascular coagulation, often manifesting cardiovascular complications. The complex mechanism remains unclear. Nor is the effective anticoagulation well established. The search for arresting hypercoagulation is of antithrombotic relevance. The ability of polybrene (PB) to inhibit tissue factor (TF)-initiated extrinsic blood coagulation was demonstrated at the protein and cellular levels as well as in human plasma samples. In a single-stage clotting assay, PB dose-dependently offset bacterial endotoxin (lipopolysaccharide)-induced monocytic TF (mTF) hypercoagulation and inhibited rabbit brain thromboplastin (rbTF) procoagulation. Consistent with these findings, the significantly prolonged prothrombin time indicated the depressed extrinsic coagulation by PB. However, PB showed no effect on thrombin time. We dissected the extrinsic pathway to further determine the inhibitory site(s) of PB. A two-stage chromogenic assay monitoring S-2288 hydrolysis showed that PB readily blocked mTF-dependent or rbTF-dependent FVII activation, which was verified by the diminished activated factor VII (FVIIa) formation derived from the proteolytic cleavage of its zymogen factor VII on Western blotting analyses. PB had no effect on FVIIa and activated factor X amidolytic activity. Nor was the dissected TF/FVIIa-catalyzed factor X activation affected. In conclusion, the preferential downregulation of factor VII activation was responsible for the depressed extrinsic coagulation. PB could present a novel anticoagulant antagonizing the extrinsic hypercoagulation for the prevention of thrombotic complication following sepsis and inflammations.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume13
Issue number2
DOIs
StatePublished - Mar 1 2002

Fingerprint

Hexadimethrine Bromide
Thromboplastin
Endotoxins
Anticoagulants
isoleucyl-prolyl-arginine-4-nitroanilide
Inflammation
Thrombin Time
Factor VIIa
Factor X
Factor VII
Factor Xa
Disseminated Intravascular Coagulation
Prothrombin Time
Blood Coagulation
Lipopolysaccharides
Sepsis
Hydrolysis
Down-Regulation
Western Blotting
Rabbits

Keywords

  • Endotoxin
  • Extrinsic coagulation
  • Factor VII activation
  • Monocytes
  • Polybrene
  • Sepsis
  • Tissue factor

ASJC Scopus subject areas

  • Hematology

Cite this

Novel anticoagulant activity of polybrene : Inhibition of monocytic tissue factor hypercoagulation following bacterial endotoxin induction. / Chu, Arthur J.; Rauci, M.; Nwobi, Obioma Ikechukwu; Mathews, S. T.; Beydoun, S.

In: Blood Coagulation and Fibrinolysis, Vol. 13, No. 2, 01.03.2002, p. 123-128.

Research output: Contribution to journalArticle

Chu, Arthur J. ; Rauci, M. ; Nwobi, Obioma Ikechukwu ; Mathews, S. T. ; Beydoun, S. / Novel anticoagulant activity of polybrene : Inhibition of monocytic tissue factor hypercoagulation following bacterial endotoxin induction. In: Blood Coagulation and Fibrinolysis. 2002 ; Vol. 13, No. 2. pp. 123-128.
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