Novel method of single antibiotic selection of cells containing multiple stage-specific promoter-fluorophore plasmids

Achuta K. Guddati, John A. Kesslet

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

This study demonstrates a novel method by which multiple separate plasmids can be stably integrated into the genome using single antibiotic resistance for selection. This method was used to integrate three different cardiac-specific promoters driving different fluorophores into murine embryonic stem cells, allowing sequential visualization of various stages of cardiac differentiation. This method is broadly applicable to the study of cell lineage of different stem/progenitor cells.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalJournal of Biomolecular Techniques
Volume21
Issue number1
Publication statusPublished - Apr 1 2010
Externally publishedYes

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Keywords

  • Differentiation

ASJC Scopus subject areas

  • Molecular Biology

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