Novel method of single antibiotic selection of cells containing multiple stage-specific promoter-fluorophore plasmids

Achuta K. Guddati, John A. Kesslet

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study demonstrates a novel method by which multiple separate plasmids can be stably integrated into the genome using single antibiotic resistance for selection. This method was used to integrate three different cardiac-specific promoters driving different fluorophores into murine embryonic stem cells, allowing sequential visualization of various stages of cardiac differentiation. This method is broadly applicable to the study of cell lineage of different stem/progenitor cells.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalJournal of Biomolecular Techniques
Volume21
Issue number1
StatePublished - Apr 1 2010
Externally publishedYes

Fingerprint

Plasmids
Anti-Bacterial Agents
Stem Cells
Cell Lineage
Embryonic Stem Cells
Microbial Drug Resistance
Genome

Keywords

  • Differentiation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Novel method of single antibiotic selection of cells containing multiple stage-specific promoter-fluorophore plasmids. / Guddati, Achuta K.; Kesslet, John A.

In: Journal of Biomolecular Techniques, Vol. 21, No. 1, 01.04.2010, p. 18-24.

Research output: Contribution to journalArticle

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