Novel multifunctional nanocomposite for root caries restorations to inhibit periodontitis-related pathogens

Shimeng Xiao, Haohao Wang, Kunneng Liang, Franklin Chi Meng Tay, Michael D. Weir, Mary Anne S. Melo, Lin Wang, Yafei Wu, Thomas W. Oates, Yi Ding, Hockin H.K. Xu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: The objectives of this study were to: (1) develop a novel multifunctional composite with nanoparticles of silver (NAg), 2-methacryloyloxyethyl phosphorylcholine (MPC), dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate biofilm-inhibition via the multifunctional nanocomposite against three species of periodontal pathogens for the first time. Methods: The multifunctional nanocomposite was fabricated by incorporating NAg, MPC, DMAHDM and NACP into the resin consisting of pyromellitic glycerol dimethacrylate (PMDGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA). Three species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for metabolic activity (MTT), live/dead staining, polysaccharide production and colony-forming units (CFU) of biofilms grown on resins. Results: Incorporation of 0.08% to 0.12% NAg, 3% MPC, 3% DMAHDM and 30% NACP did not compromise the mechanical properties of the composite (p > 0.1). The multifunctional nanocomposite reduced protein adsorption to nearly 1/10 of that of a commercial control (p < 0.05). For all three species, the biofilm CFU was reduced by about 5 and 1 orders of magnitude via the nanocomposite containing NAg + MPC + DMAHDM, compared to commercial control and the composite with MPC + DMAHDM, respectively. Conclusions: The novel multifunctional nanocomposite achieved the greatest reduction in metabolic activity, polysaccharide and biofilm growth of three periodontal pathogens. Clinical significance: The strongly-antibacterial, multifunctional composite is promising for treating root lesions, alleviating periodontitis and protecting the periodontal tissues.

Original languageEnglish (US)
Pages (from-to)17-26
Number of pages10
JournalJournal of Dentistry
Volume81
DOIs
StatePublished - Feb 1 2019

Fingerprint

Root Caries
Nanocomposites
Methacrylates
Periodontitis
Biofilms
Nanoparticles
Polysaccharides
Stem Cells
Fusobacterium nucleatum
Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Silver
Glycerol
Adsorption
2-methacryloyloxyethyl phosphorylcholine
Staining and Labeling
Growth
amorphous calcium phosphate
Proteins

Keywords

  • Antibacterial activity
  • Class V restorations
  • Multifunctional nanocomposite
  • Periodontal pathogens
  • Root caries
  • Silver nanoparticle

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Novel multifunctional nanocomposite for root caries restorations to inhibit periodontitis-related pathogens. / Xiao, Shimeng; Wang, Haohao; Liang, Kunneng; Tay, Franklin Chi Meng; Weir, Michael D.; Melo, Mary Anne S.; Wang, Lin; Wu, Yafei; Oates, Thomas W.; Ding, Yi; Xu, Hockin H.K.

In: Journal of Dentistry, Vol. 81, 01.02.2019, p. 17-26.

Research output: Contribution to journalArticle

Xiao, S, Wang, H, Liang, K, Tay, FCM, Weir, MD, Melo, MAS, Wang, L, Wu, Y, Oates, TW, Ding, Y & Xu, HHK 2019, 'Novel multifunctional nanocomposite for root caries restorations to inhibit periodontitis-related pathogens', Journal of Dentistry, vol. 81, pp. 17-26. https://doi.org/10.1016/j.jdent.2018.12.001
Xiao, Shimeng ; Wang, Haohao ; Liang, Kunneng ; Tay, Franklin Chi Meng ; Weir, Michael D. ; Melo, Mary Anne S. ; Wang, Lin ; Wu, Yafei ; Oates, Thomas W. ; Ding, Yi ; Xu, Hockin H.K. / Novel multifunctional nanocomposite for root caries restorations to inhibit periodontitis-related pathogens. In: Journal of Dentistry. 2019 ; Vol. 81. pp. 17-26.
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author = "Shimeng Xiao and Haohao Wang and Kunneng Liang and Tay, {Franklin Chi Meng} and Weir, {Michael D.} and Melo, {Mary Anne S.} and Lin Wang and Yafei Wu and Oates, {Thomas W.} and Yi Ding and Xu, {Hockin H.K.}",
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AU - Xiao, Shimeng

AU - Wang, Haohao

AU - Liang, Kunneng

AU - Tay, Franklin Chi Meng

AU - Weir, Michael D.

AU - Melo, Mary Anne S.

AU - Wang, Lin

AU - Wu, Yafei

AU - Oates, Thomas W.

AU - Ding, Yi

AU - Xu, Hockin H.K.

PY - 2019/2/1

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N2 - Objectives: The objectives of this study were to: (1) develop a novel multifunctional composite with nanoparticles of silver (NAg), 2-methacryloyloxyethyl phosphorylcholine (MPC), dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate biofilm-inhibition via the multifunctional nanocomposite against three species of periodontal pathogens for the first time. Methods: The multifunctional nanocomposite was fabricated by incorporating NAg, MPC, DMAHDM and NACP into the resin consisting of pyromellitic glycerol dimethacrylate (PMDGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA). Three species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for metabolic activity (MTT), live/dead staining, polysaccharide production and colony-forming units (CFU) of biofilms grown on resins. Results: Incorporation of 0.08% to 0.12% NAg, 3% MPC, 3% DMAHDM and 30% NACP did not compromise the mechanical properties of the composite (p > 0.1). The multifunctional nanocomposite reduced protein adsorption to nearly 1/10 of that of a commercial control (p < 0.05). For all three species, the biofilm CFU was reduced by about 5 and 1 orders of magnitude via the nanocomposite containing NAg + MPC + DMAHDM, compared to commercial control and the composite with MPC + DMAHDM, respectively. Conclusions: The novel multifunctional nanocomposite achieved the greatest reduction in metabolic activity, polysaccharide and biofilm growth of three periodontal pathogens. Clinical significance: The strongly-antibacterial, multifunctional composite is promising for treating root lesions, alleviating periodontitis and protecting the periodontal tissues.

AB - Objectives: The objectives of this study were to: (1) develop a novel multifunctional composite with nanoparticles of silver (NAg), 2-methacryloyloxyethyl phosphorylcholine (MPC), dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate biofilm-inhibition via the multifunctional nanocomposite against three species of periodontal pathogens for the first time. Methods: The multifunctional nanocomposite was fabricated by incorporating NAg, MPC, DMAHDM and NACP into the resin consisting of pyromellitic glycerol dimethacrylate (PMDGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA). Three species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for metabolic activity (MTT), live/dead staining, polysaccharide production and colony-forming units (CFU) of biofilms grown on resins. Results: Incorporation of 0.08% to 0.12% NAg, 3% MPC, 3% DMAHDM and 30% NACP did not compromise the mechanical properties of the composite (p > 0.1). The multifunctional nanocomposite reduced protein adsorption to nearly 1/10 of that of a commercial control (p < 0.05). For all three species, the biofilm CFU was reduced by about 5 and 1 orders of magnitude via the nanocomposite containing NAg + MPC + DMAHDM, compared to commercial control and the composite with MPC + DMAHDM, respectively. Conclusions: The novel multifunctional nanocomposite achieved the greatest reduction in metabolic activity, polysaccharide and biofilm growth of three periodontal pathogens. Clinical significance: The strongly-antibacterial, multifunctional composite is promising for treating root lesions, alleviating periodontitis and protecting the periodontal tissues.

KW - Antibacterial activity

KW - Class V restorations

KW - Multifunctional nanocomposite

KW - Periodontal pathogens

KW - Root caries

KW - Silver nanoparticle

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