Novel nanotechnology and near-infrared photodynamic therapy to kill periodontitis-related biofilm pathogens and protect the periodontium

Manlin Qi, Xue Li, Xiaolin Sun, Chunyan Li, Franklin R. Tay, Michael D. Weir, Biao Dong, Yanmin Zhou, Lin Wang, Hockin H.K. Xu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective: Periodontal tissue destruction and tooth loss are increasingly a worldwide problem as the population ages. Periodontitis is caused by bacterial infection and biofilm plaque buildup. Therefore, the objectives of this study were to: (1) develop a near-infrared light (NIR)-triggered core-shell nanostructure of upconversion nanoparticles and TiO2 (UCNPs@TiO2), and (2) investigate its inhibitory effects via antibacterial photodynamic therapy (aPDT) against periodontitis-related pathogens. Methods: The core β-NaYF4:Yb3+,Tm3+ were synthesized via thermal decomposition and further modified with the TiO2 shell via a hydrothermal method. The core-shell structure and the upconversion fluorescence-induced aPDT treatment via 980 nm laser were studied. Three periodontitis-related pathogens Streptococcus sanguinis (S. sanguinis), Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum) were investigated. The killing activity against planktonic bacteria was detected by a time-kill assay. Single species 4-day biofilms on dentin were tested by live/dead staining, colony-forming units (CFU), and metabolic activity. Results: The hexagonal shaped UCNPs@TiO2 had an average diameter of 39.7 nm. UCNPs@TiO2 nanoparticles had positively charged (+12.4 mV) surface and were biocompatible and non-cytotoxic. Under the excitation of NIR light (980 nm), the core NaYF4:Yb3+,Tm3+ UCNPs could emit intense ultraviolet (UV) light, which further triggered the aPDT function of the shell TiO2 via energy transfer, thereby realizing the remarkable antibacterial effects against planktons and biofilms of periodontitis-associated pathogens. NIR-triggered UCNPs@TiO2 achieved much greater reduction in biofilms than control (p < 0.05). Biofilm CFU was reduced by 3–4 orders of magnitude via NIR-triggered aPDT, which is significantly greater than that of negative control and commercial aPDT control groups. The killing efficacy of UCNPs@TiO2-based aPDT against the three species was ranked to be: S. sanguinis < F. nucleatum = P. gingivalis. Metabolic activities of biofilms were also greatly reduced via NIR-triggered aPDT (p < 0.05). Significance: Upconversion fluorescence-based aPDT achieved strong inhibiting effects against all three species of periodontitis-related pathogens. This novel nanotechnology demonstrated a high promise to inhibit periodontitis, with exciting potential to combat other oral infectious diseases such as deep endodontic infections.

Original languageEnglish (US)
Pages (from-to)1665-1681
Number of pages17
JournalDental Materials
Volume35
Issue number11
DOIs
StatePublished - Nov 2019

Keywords

  • Antibacterial
  • Near-infrared
  • Periodontitis biofilms
  • Photodynamic therapy
  • Titanium dioxide
  • Upconversion nanoparticles

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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