NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli

Daria V. Ilatovskaya, Vladislav Levchenko, Robert P. Ryan, Allen W. Cowley, Alexander Staruschenko

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.

Original languageEnglish (US)
Pages (from-to)242-247
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume408
Issue number2
DOIs
StatePublished - May 6 2011
Externally publishedYes

Keywords

  • Focal segmental glomerulosclerosis
  • FSGS
  • Podocyte
  • TRPC6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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