Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells

Ravi Jotwani, Baljit S. Moonga, Siddharth Gupta, Christopher W Cutler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

The pathogenesis of a number of chronic inflammatory diseases has been linked to dysregulated functioning of nuclear transcription factor (NF)-κB. In the present study, we examine NF-κB activation in human oral mucosal tissues of chronic periodontitis (CP) patients. Electrophoretic mobility shift assay and DNA-binding enzyme-linked immunosorbent assays indicate elevated levels of transcriptionally repressive p50 subunits and an increased p50/p65 ratio in CP tissues compared to healthy controls. Because Porphyromonas gingivalis has been recognized to be a causal factor in CP, we used P. gingivalis lipopolysaccharide (LPS) for in vitro studies with monocyte-derived dendritic cells (MoDCs). Porphyromonas gingivalis LPS, unlike Escherichia coli LPS, induced an increased p50/p65 ratio in MoDCs. Using blocking antibodies, we demonstrated that while both Toll-like receptor 2 (TLR2) and TLR4 are required for MoDC maturation by P. gingivalis LPS, only TLR4 signaling is sufficient to induce cytokine secretion. Our results suggest that increased levels of transcriptionally repressive p50 may be characteristic of CP and might be a result of suboptimal NF-κB activation and dendritic cell maturation by P. gingivalis, a bacterium implicated in CP.

Original languageEnglish (US)
Title of host publicationSkeletal Biology and Medicine
PublisherBlackwell Publishing Inc.
Pages278-285
Number of pages8
ISBN (Print)9781573317856
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1192
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Chronic Periodontitis
Porphyromonas gingivalis
Dendritic Cells
Lipopolysaccharides
Transcription Factors
Monocytes
Assays
Chemical activation
Toll-Like Receptor 2
Electrophoretic mobility
Immunosorbents
Blocking Antibodies
Escherichia coli
Electrophoretic Mobility Shift Assay
Bacteria
Tissue
Cytokines
Mucous Membrane
Chronic Disease
Enzyme-Linked Immunosorbent Assay

Keywords

  • Dendritic cells
  • LPS
  • NF-κB
  • P50/p65
  • Periodontitis
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Jotwani, R., Moonga, B. S., Gupta, S., & Cutler, C. W. (2010). Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells. In Skeletal Biology and Medicine (pp. 278-285). (Annals of the New York Academy of Sciences; Vol. 1192). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.05247.x

Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells. / Jotwani, Ravi; Moonga, Baljit S.; Gupta, Siddharth; Cutler, Christopher W.

Skeletal Biology and Medicine. Blackwell Publishing Inc., 2010. p. 278-285 (Annals of the New York Academy of Sciences; Vol. 1192).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jotwani, R, Moonga, BS, Gupta, S & Cutler, CW 2010, Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells. in Skeletal Biology and Medicine. Annals of the New York Academy of Sciences, vol. 1192, Blackwell Publishing Inc., pp. 278-285. https://doi.org/10.1111/j.1749-6632.2009.05247.x
Jotwani R, Moonga BS, Gupta S, Cutler CW. Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells. In Skeletal Biology and Medicine. Blackwell Publishing Inc. 2010. p. 278-285. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.05247.x
Jotwani, Ravi ; Moonga, Baljit S. ; Gupta, Siddharth ; Cutler, Christopher W. / Nuclear factor-κB p50 subunits in chronic periodontitis and Porphyromonas gingivalis lipopolysaccharide-pulsed dendritic cells. Skeletal Biology and Medicine. Blackwell Publishing Inc., 2010. pp. 278-285 (Annals of the New York Academy of Sciences).
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