Nucleobase-based barbiturates: Their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay

Bhaveshkumar D. Dhorajiya, Bharatkumar Z. Dholakiya, Ahmed S. Ibrahim, Farid A. Badria

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, 1H NMR, 13C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds TBC > TBA > TBG showed highest lymphocyte transformation assay, TBC > TBA > BG showed inhibitory antioxidant activity using ABTS methods, and TBC > BPA > BAMT > TBA > 1, 3 -TBA manifested the best protective effect against DNA damage induced by bleomycin.

Original languageEnglish (US)
Article number898670
JournalBioMed Research International
Volume2014
DOIs
StatePublished - 2014

Fingerprint

Barbiturates
Lymphocytes
Bleomycin
Lymphocyte Activation
DNA Damage
Heterocyclic Acids
Assays
Antioxidants
DNA
Ultraviolet spectroscopy
Spectrum analysis
Nucleic Acids
Nuclear magnetic resonance spectroscopy
Amines
Spectrum Analysis
Nuclear magnetic resonance
Derivatives
Chemical analysis
iron bleomycin
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Nucleobase-based barbiturates : Their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay. / Dhorajiya, Bhaveshkumar D.; Dholakiya, Bharatkumar Z.; Ibrahim, Ahmed S.; Badria, Farid A.

In: BioMed Research International, Vol. 2014, 898670, 2014.

Research output: Contribution to journalArticle

Dhorajiya, Bhaveshkumar D. ; Dholakiya, Bharatkumar Z. ; Ibrahim, Ahmed S. ; Badria, Farid A. / Nucleobase-based barbiturates : Their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay. In: BioMed Research International. 2014 ; Vol. 2014.
@article{dd8989267f9d469fbd06af39c1f72e04,
title = "Nucleobase-based barbiturates: Their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay",
abstract = "A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, 1H NMR, 13C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds TBC > TBA > TBG showed highest lymphocyte transformation assay, TBC > TBA > BG showed inhibitory antioxidant activity using ABTS methods, and TBC > BPA > BAMT > TBA > 1, 3 -TBA manifested the best protective effect against DNA damage induced by bleomycin.",
author = "Dhorajiya, {Bhaveshkumar D.} and Dholakiya, {Bharatkumar Z.} and Ibrahim, {Ahmed S.} and Badria, {Farid A.}",
year = "2014",
doi = "10.1155/2014/898670",
language = "English (US)",
volume = "2014",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Nucleobase-based barbiturates

T2 - Their protective effect against DNA damage induced by bleomycin-iron, antioxidant, and lymphocyte transformation assay

AU - Dhorajiya, Bhaveshkumar D.

AU - Dholakiya, Bharatkumar Z.

AU - Ibrahim, Ahmed S.

AU - Badria, Farid A.

PY - 2014

Y1 - 2014

N2 - A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, 1H NMR, 13C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds TBC > TBA > TBG showed highest lymphocyte transformation assay, TBC > TBA > BG showed inhibitory antioxidant activity using ABTS methods, and TBC > BPA > BAMT > TBA > 1, 3 -TBA manifested the best protective effect against DNA damage induced by bleomycin.

AB - A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, 1H NMR, 13C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds TBC > TBA > TBG showed highest lymphocyte transformation assay, TBC > TBA > BG showed inhibitory antioxidant activity using ABTS methods, and TBC > BPA > BAMT > TBA > 1, 3 -TBA manifested the best protective effect against DNA damage induced by bleomycin.

UR - http://www.scopus.com/inward/record.url?scp=84901771878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901771878&partnerID=8YFLogxK

U2 - 10.1155/2014/898670

DO - 10.1155/2014/898670

M3 - Article

C2 - 24900997

AN - SCOPUS:84901771878

VL - 2014

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 898670

ER -