Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes

Dmitri Dozortsev, Allen Coleman, Peter Nagy, Michael P. Diamond, Alexandre Ermilov, Ulli Weier, Marek Liyanage, Thomas Reid

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.

Original languageEnglish (US)
Pages (from-to)366-371
Number of pages6
JournalFertility and sterility
Volume73
Issue number2
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

Fingerprint

Satellite DNA
Embryonic Structures
Chromosomes
Centromere
Interphase
Paintings
Zygote
Telomere
Outcome Assessment (Health Care)
DNA

Keywords

  • Centromeres
  • Nucleoli
  • Telomeres
  • Zygote
  • α-satellite

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes. / Dozortsev, Dmitri; Coleman, Allen; Nagy, Peter; Diamond, Michael P.; Ermilov, Alexandre; Weier, Ulli; Liyanage, Marek; Reid, Thomas.

In: Fertility and sterility, Vol. 73, No. 2, 01.02.2000, p. 366-371.

Research output: Contribution to journalArticle

Dozortsev, Dmitri ; Coleman, Allen ; Nagy, Peter ; Diamond, Michael P. ; Ermilov, Alexandre ; Weier, Ulli ; Liyanage, Marek ; Reid, Thomas. / Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes. In: Fertility and sterility. 2000 ; Vol. 73, No. 2. pp. 366-371.
@article{d8a00ec03fc74afa9e1fe5110a52fe5b,
title = "Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes",
abstract = "Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.",
keywords = "Centromeres, Nucleoli, Telomeres, Zygote, α-satellite",
author = "Dmitri Dozortsev and Allen Coleman and Peter Nagy and Diamond, {Michael P.} and Alexandre Ermilov and Ulli Weier and Marek Liyanage and Thomas Reid",
year = "2000",
month = "2",
day = "1",
doi = "10.1016/S0015-0282(99)00491-4",
language = "English (US)",
volume = "73",
pages = "366--371",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes

AU - Dozortsev, Dmitri

AU - Coleman, Allen

AU - Nagy, Peter

AU - Diamond, Michael P.

AU - Ermilov, Alexandre

AU - Weier, Ulli

AU - Liyanage, Marek

AU - Reid, Thomas

PY - 2000/2/1

Y1 - 2000/2/1

N2 - Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.

AB - Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.

KW - Centromeres

KW - Nucleoli

KW - Telomeres

KW - Zygote

KW - α-satellite

UR - http://www.scopus.com/inward/record.url?scp=0033977692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033977692&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(99)00491-4

DO - 10.1016/S0015-0282(99)00491-4

M3 - Article

C2 - 10685545

AN - SCOPUS:0033977692

VL - 73

SP - 366

EP - 371

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 2

ER -