Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes

Dmitri Dozortsev, Allen Coleman, Peter Nagy, Michael P. Diamond, Alexandre Ermilov, Ulli Weier, Marek Liyanage, Thomas Reid

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.

Original languageEnglish (US)
Pages (from-to)366-371
Number of pages6
JournalFertility and sterility
Volume73
Issue number2
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

Keywords

  • Centromeres
  • Nucleoli
  • Telomeres
  • Zygote
  • α-satellite

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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