Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase

P. S. Henthorn, M. Raducha, Y. H. Edwards, M. J. Weiss, C. Slaughter, M. A. Lafferty, H. Harris

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

A cDNA probe for human adult intestinal alkaline phosphatase (ALP) [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] was isolated from a λgt11 expression library. The cDNA insert of this close is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of human intestinal ALP, as determined by direct protein sequencing. Intestinal ALP shows 86.5% amino acid identity to placental (type 1) ALP and 56.6% amino acid identity to liver/bone/kidney ALP. In the 3'-untranslated regions, intestinal and placental ALP cDNAs are 73.5% identical (excluding gaps). The evolution of this multigene enzyme family is discussed.

Original languageEnglish (US)
Pages (from-to)1234-1238
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number5
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

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