Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase

P. S. Henthorn, M. Raducha, Y. H. Edwards, M. J. Weiss, C. Slaughter, M. A. Lafferty, H. Harris

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

A cDNA probe for human adult intestinal alkaline phosphatase (ALP) [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] was isolated from a λgt11 expression library. The cDNA insert of this close is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of human intestinal ALP, as determined by direct protein sequencing. Intestinal ALP shows 86.5% amino acid identity to placental (type 1) ALP and 56.6% amino acid identity to liver/bone/kidney ALP. In the 3'-untranslated regions, intestinal and placental ALP cDNAs are 73.5% identical (excluding gaps). The evolution of this multigene enzyme family is discussed.

Original languageEnglish (US)
Pages (from-to)1234-1238
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number5
DOIs
StatePublished - Apr 29 1987

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Amino Acid Sequence
Nucleotides
Alkaline Phosphatase
Amino Acids
Complementary DNA
Peptides
Protein Sequence Analysis
3' Untranslated Regions
Multigene Family
Base Pairing
Open Reading Frames
Kidney
Bone and Bones
human ALPI protein
placental alkaline phosphatase
Liver
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Nucleotide and amino acid sequences of human intestinal alkaline phosphatase : Close homology to placental alkaline phosphatase. / Henthorn, P. S.; Raducha, M.; Edwards, Y. H.; Weiss, M. J.; Slaughter, C.; Lafferty, M. A.; Harris, H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 5, 29.04.1987, p. 1234-1238.

Research output: Contribution to journalArticle

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AU - Edwards, Y. H.

AU - Weiss, M. J.

AU - Slaughter, C.

AU - Lafferty, M. A.

AU - Harris, H.

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