Nucleotide sequencing and PCR-RFLP of insulin-like growth factor binding protein-3 gene in riverine buffalo (Bubalus bubalis)

B. Padma, Pushpendra Kumar, Vivek Choudhary, S. K. Dhara, A. Mishra, T. K. Bhattacharya, B. Bhushan, Arjava Sharma

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reaction-restriction fragment polymorphism (PCR-RPLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows non-polymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle.

Original languageEnglish (US)
Pages (from-to)910-913
Number of pages4
JournalAsian-Australasian Journal of Animal Sciences
Volume17
Issue number7
DOIs
StatePublished - Jan 1 2004

Fingerprint

Insulin-Like Growth Factor Binding Protein 3
insulin-like growth factor binding proteins
Buffaloes
Restriction Fragment Length Polymorphisms
buffaloes
restriction fragment length polymorphism
Nucleotides
nucleotides
Polymerase Chain Reaction
Genes
genes
breeds
animals
structural genes
Nucleic Acid Databases
growth and development
Growth and Development
digestion
polymerase chain reaction
genetic polymorphism

Keywords

  • Buffalo
  • IGFBP-3
  • PCR-RPLP
  • Polymorphism
  • Sequencing

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

Cite this

Nucleotide sequencing and PCR-RFLP of insulin-like growth factor binding protein-3 gene in riverine buffalo (Bubalus bubalis). / Padma, B.; Kumar, Pushpendra; Choudhary, Vivek; Dhara, S. K.; Mishra, A.; Bhattacharya, T. K.; Bhushan, B.; Sharma, Arjava.

In: Asian-Australasian Journal of Animal Sciences, Vol. 17, No. 7, 01.01.2004, p. 910-913.

Research output: Contribution to journalArticle

Padma, B. ; Kumar, Pushpendra ; Choudhary, Vivek ; Dhara, S. K. ; Mishra, A. ; Bhattacharya, T. K. ; Bhushan, B. ; Sharma, Arjava. / Nucleotide sequencing and PCR-RFLP of insulin-like growth factor binding protein-3 gene in riverine buffalo (Bubalus bubalis). In: Asian-Australasian Journal of Animal Sciences. 2004 ; Vol. 17, No. 7. pp. 910-913.
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