Nucleotide sequencing and PCR-RFLP of insulin-like growth factor binding protein-3 gene in riverine buffalo (Bubalus bubalis)

B. Padma, Pushpendra Kumar, Vivek Choudhary, S. K. Dhara, A. Mishra, T. K. Bhattacharya, B. Bhushan, Arjava Sharma

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reaction-restriction fragment polymorphism (PCR-RPLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows non-polymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle.

Original languageEnglish (US)
Pages (from-to)910-913
Number of pages4
JournalAsian-Australasian Journal of Animal Sciences
Volume17
Issue number7
DOIs
StatePublished - Jul 2004

Keywords

  • Buffalo
  • IGFBP-3
  • PCR-RPLP
  • Polymorphism
  • Sequencing

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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