Observation of cellular cholesterol efflux at microcavity electrodes

Danjun Fang, Dechen Jiang, Hui Lu, Hillel J. Chiel, Thomas J. Kelley, James D. Burgess

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

(Figure Presented) Cholesterol oxidase modified platinum microcavity electrodes are used to measure cholesterol efflux from the plasma membrane surface of a single neuron in the buccal ganglion of Aplysia at room temperature. A background subtraction analog chronocoulometry method is used to measure hydrogen peroxide accumulation in the microcavity volume resulting from cellular cholesterol efflux and enzymatic oxidation. The data are consistent with the aqueous diffusion model for cellular cholesterol efflux where plasma membrane cholesterol undergoes exchange with solution phase cholesterol.

Original languageEnglish (US)
Pages (from-to)12038-12039
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number34
DOIs
StatePublished - Sep 2 2009

Fingerprint

Microcavities
Cholesterol
Electrodes
Observation
Cell membranes
Cholesterol Oxidase
Cell Membrane
Aplysia
Cheek
Platinum
Ganglia
Hydrogen Peroxide
Neurons
Ion exchange
Hydrogen peroxide
Oxidation
Temperature

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Fang, D., Jiang, D., Lu, H., Chiel, H. J., Kelley, T. J., & Burgess, J. D. (2009). Observation of cellular cholesterol efflux at microcavity electrodes. Journal of the American Chemical Society, 131(34), 12038-12039. https://doi.org/10.1021/ja903684f

Observation of cellular cholesterol efflux at microcavity electrodes. / Fang, Danjun; Jiang, Dechen; Lu, Hui; Chiel, Hillel J.; Kelley, Thomas J.; Burgess, James D.

In: Journal of the American Chemical Society, Vol. 131, No. 34, 02.09.2009, p. 12038-12039.

Research output: Contribution to journalArticle

Fang, D, Jiang, D, Lu, H, Chiel, HJ, Kelley, TJ & Burgess, JD 2009, 'Observation of cellular cholesterol efflux at microcavity electrodes', Journal of the American Chemical Society, vol. 131, no. 34, pp. 12038-12039. https://doi.org/10.1021/ja903684f
Fang, Danjun ; Jiang, Dechen ; Lu, Hui ; Chiel, Hillel J. ; Kelley, Thomas J. ; Burgess, James D. / Observation of cellular cholesterol efflux at microcavity electrodes. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 34. pp. 12038-12039.
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