Observation of cellular cholesterol efflux at microcavity electrodes

Danjun Fang, Dechen Jiang, Hui Lu, Hillel J. Chiel, Thomas J. Kelley, James D. Burgess

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

(Figure Presented) Cholesterol oxidase modified platinum microcavity electrodes are used to measure cholesterol efflux from the plasma membrane surface of a single neuron in the buccal ganglion of Aplysia at room temperature. A background subtraction analog chronocoulometry method is used to measure hydrogen peroxide accumulation in the microcavity volume resulting from cellular cholesterol efflux and enzymatic oxidation. The data are consistent with the aqueous diffusion model for cellular cholesterol efflux where plasma membrane cholesterol undergoes exchange with solution phase cholesterol.

Original languageEnglish (US)
Pages (from-to)12038-12039
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number34
DOIs
StatePublished - Sep 2 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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