Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions

Gianluca Turco, Andrea Frassetto, Luca Fontanive, Annalisa Mazzoni, Milena Cadenaro, Roberto Di Lenarda, Franklin Chi Meng Tay, David Henry Pashley, Lorenzo Breschi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective This study evaluated the ability of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) to improve the stability of demineralized dentin collagen matrices when subjected to mechanical cycling by means of Chewing Simulation (CS). Methods Demineralized dentin disks were randomly assigned to four groups (N = 4): (1) immersion in artificial saliva at 37 °C for 30 days; (2) pre-treatment with 0.5 M EDC for 60 s, then stored as in Group 1; (3) CS challenge (50 N occlusal load, 30 s occlusal time plus 30 s with no load, for 30 days); (4) pre-treatment with 0.5 M EDC as in Group 2 and CS challenge as in Group 3. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptides. Results EDC treated specimens showed no significant telopeptides release, irrespective of the aging method. Cyclic stressing of EDC-untreated specimens caused significantly higher ICTP release at day 1, compared to static storage, while by days 3 and 4, the ICTP release in the cyclic group fell significantly below the static group, and then remained undetectable from 5 to 30 days. CTX release in the cyclic groups, on EDC-untreated control specimens was always lower than in the static group in days 1-4, and then fell to undetectable for 30 days. Significance This study showed that chewing stresses applied to control untreated demineralized dentin increased degradation of collagen in terms of CTX release, while collagen crosslinking agents may prevent dentin collagen degradation, irrespective of simulated occlusal function.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalDental Materials
Volume32
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Mastication
Dentin
Collagen
Degradation
Ethyldimethylaminopropyl Carbodiimide
Artificial Saliva
Immersion
Crosslinking
Aging of materials
Sampling

Keywords

  • Collagen(s)
  • Cross-linking agent
  • Demineralization
  • Dentin
  • Mastication Stress
  • Matrix metalloproteinases (MMPs)

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Turco, G., Frassetto, A., Fontanive, L., Mazzoni, A., Cadenaro, M., Di Lenarda, R., ... Breschi, L. (2016). Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions. Dental Materials, 32(2), 192-199. https://doi.org/10.1016/j.dental.2015.11.026

Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions. / Turco, Gianluca; Frassetto, Andrea; Fontanive, Luca; Mazzoni, Annalisa; Cadenaro, Milena; Di Lenarda, Roberto; Tay, Franklin Chi Meng; Pashley, David Henry; Breschi, Lorenzo.

In: Dental Materials, Vol. 32, No. 2, 01.02.2016, p. 192-199.

Research output: Contribution to journalArticle

Turco, G, Frassetto, A, Fontanive, L, Mazzoni, A, Cadenaro, M, Di Lenarda, R, Tay, FCM, Pashley, DH & Breschi, L 2016, 'Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions', Dental Materials, vol. 32, no. 2, pp. 192-199. https://doi.org/10.1016/j.dental.2015.11.026
Turco G, Frassetto A, Fontanive L, Mazzoni A, Cadenaro M, Di Lenarda R et al. Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions. Dental Materials. 2016 Feb 1;32(2):192-199. https://doi.org/10.1016/j.dental.2015.11.026
Turco, Gianluca ; Frassetto, Andrea ; Fontanive, Luca ; Mazzoni, Annalisa ; Cadenaro, Milena ; Di Lenarda, Roberto ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Breschi, Lorenzo. / Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions. In: Dental Materials. 2016 ; Vol. 32, No. 2. pp. 192-199.
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abstract = "Objective This study evaluated the ability of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) to improve the stability of demineralized dentin collagen matrices when subjected to mechanical cycling by means of Chewing Simulation (CS). Methods Demineralized dentin disks were randomly assigned to four groups (N = 4): (1) immersion in artificial saliva at 37 °C for 30 days; (2) pre-treatment with 0.5 M EDC for 60 s, then stored as in Group 1; (3) CS challenge (50 N occlusal load, 30 s occlusal time plus 30 s with no load, for 30 days); (4) pre-treatment with 0.5 M EDC as in Group 2 and CS challenge as in Group 3. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptides. Results EDC treated specimens showed no significant telopeptides release, irrespective of the aging method. Cyclic stressing of EDC-untreated specimens caused significantly higher ICTP release at day 1, compared to static storage, while by days 3 and 4, the ICTP release in the cyclic group fell significantly below the static group, and then remained undetectable from 5 to 30 days. CTX release in the cyclic groups, on EDC-untreated control specimens was always lower than in the static group in days 1-4, and then fell to undetectable for 30 days. Significance This study showed that chewing stresses applied to control untreated demineralized dentin increased degradation of collagen in terms of CTX release, while collagen crosslinking agents may prevent dentin collagen degradation, irrespective of simulated occlusal function.",
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N2 - Objective This study evaluated the ability of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) to improve the stability of demineralized dentin collagen matrices when subjected to mechanical cycling by means of Chewing Simulation (CS). Methods Demineralized dentin disks were randomly assigned to four groups (N = 4): (1) immersion in artificial saliva at 37 °C for 30 days; (2) pre-treatment with 0.5 M EDC for 60 s, then stored as in Group 1; (3) CS challenge (50 N occlusal load, 30 s occlusal time plus 30 s with no load, for 30 days); (4) pre-treatment with 0.5 M EDC as in Group 2 and CS challenge as in Group 3. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptides. Results EDC treated specimens showed no significant telopeptides release, irrespective of the aging method. Cyclic stressing of EDC-untreated specimens caused significantly higher ICTP release at day 1, compared to static storage, while by days 3 and 4, the ICTP release in the cyclic group fell significantly below the static group, and then remained undetectable from 5 to 30 days. CTX release in the cyclic groups, on EDC-untreated control specimens was always lower than in the static group in days 1-4, and then fell to undetectable for 30 days. Significance This study showed that chewing stresses applied to control untreated demineralized dentin increased degradation of collagen in terms of CTX release, while collagen crosslinking agents may prevent dentin collagen degradation, irrespective of simulated occlusal function.

AB - Objective This study evaluated the ability of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) to improve the stability of demineralized dentin collagen matrices when subjected to mechanical cycling by means of Chewing Simulation (CS). Methods Demineralized dentin disks were randomly assigned to four groups (N = 4): (1) immersion in artificial saliva at 37 °C for 30 days; (2) pre-treatment with 0.5 M EDC for 60 s, then stored as in Group 1; (3) CS challenge (50 N occlusal load, 30 s occlusal time plus 30 s with no load, for 30 days); (4) pre-treatment with 0.5 M EDC as in Group 2 and CS challenge as in Group 3. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptides. Results EDC treated specimens showed no significant telopeptides release, irrespective of the aging method. Cyclic stressing of EDC-untreated specimens caused significantly higher ICTP release at day 1, compared to static storage, while by days 3 and 4, the ICTP release in the cyclic group fell significantly below the static group, and then remained undetectable from 5 to 30 days. CTX release in the cyclic groups, on EDC-untreated control specimens was always lower than in the static group in days 1-4, and then fell to undetectable for 30 days. Significance This study showed that chewing stresses applied to control untreated demineralized dentin increased degradation of collagen in terms of CTX release, while collagen crosslinking agents may prevent dentin collagen degradation, irrespective of simulated occlusal function.

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