Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells

Gregg T. Nagle, Walter R.A. van Heumen, Susan L. Knock, Anna T. Garcia, David A. McCullough, Alexander Kurosky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

1. 1. Strong evidence is accumulating that the endoproteases which process prohormones at dibasic residue cleavage sites are members of a subtilisin-related class of proteases. 2. 2. Using the polymerase chain reaction (PCR), we have isolated and characterized an Aplysia californica neuroendocrine bag cell cDNA product (270 base pairs) that encodes a sequence which is highly homologous to the subtilisin-related class of processing proteases that includes yeast Kex2, human/mouse/Drosophila furin, human PC2, and mouse PC1/PC3 and PC2. 3. 3. The characterized cDNA PCR product showed the highest degree of residue identity with the furin-related group of proteins (human/mouse furin 71%; Drosophila furin 63%). 4. 4. These results establish that Aplysia contain a subtilisin-like gene and suggest that the expression of this gene may play a role in processing Aplysia precursor proteins in the bag cells and likely also in the exocrine atrial gland. 5. 5. Furthermore, the Aplysia nucleotide sequence results, together with available sequence information from human, mouse, and Drosophila furins, provide reasonable evidence that the furin-like enzymes may represent a separate subclass of the subtilisin-like processing enzymes.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume105
Issue number2
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Furin
Aplysia
Neuroendocrine Cells
Subtilisin
Enzymes
Processing
Drosophila
Polymerase chain reaction
Peptide Hydrolases
Complementary DNA
Genes
Exocrine Glands
Polymerase Chain Reaction
Protein Precursors
Reaction products
Base Pairing
Yeast
Nucleotides
Yeasts
Gene Expression

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology

Cite this

Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells. / Nagle, Gregg T.; van Heumen, Walter R.A.; Knock, Susan L.; Garcia, Anna T.; McCullough, David A.; Kurosky, Alexander.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 105, No. 2, 01.01.1993, p. 345-348.

Research output: Contribution to journalArticle

Nagle, Gregg T. ; van Heumen, Walter R.A. ; Knock, Susan L. ; Garcia, Anna T. ; McCullough, David A. ; Kurosky, Alexander. / Occurrence of a furin-like prohormone processing enzyme in Aplysia neuroendocrine bag cells. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1993 ; Vol. 105, No. 2. pp. 345-348.
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