Oligoclonal β-galactoside-binding immunoglobulins antigenically related to 14kda lectin in human serum and cerebrospinal fluid: Purification and characterization

Raymonde Joubert-Caron, Michel Caron, Pascal Bochet, Ahmed Chadli, Pierre Delaporte, Edmond Schuller, Dominique Bladier

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

1. 1. An antiserum raised against a 14kDa β-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a β-galactoside-binding IgG fraction (lectin-like IgG). 2. 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to β-Gal-containing structures and close to the HBL14 one.

Original languageEnglish (US)
Pages (from-to)813-823
Number of pages11
JournalInternational Journal of Biochemistry
Volume26
Issue number6
DOIs
StatePublished - Jan 1 1994

Fingerprint

Cerebrospinal fluid
Galactosides
Lectins
Purification
Cerebrospinal Fluid
Immunoglobulins
Immunoglobulin G
Serum
Affinity chromatography
Immunoglobulin Fab Fragments
Affinity Chromatography
Immune Sera
Anti-Idiotypic Antibodies
Brain
Copolymers
Carbohydrates
Rabbits
Light
Molecules

Keywords

  • Soluble lectin Immunoglobulins Affinity chromatography Anti-idiotypes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oligoclonal β-galactoside-binding immunoglobulins antigenically related to 14kda lectin in human serum and cerebrospinal fluid : Purification and characterization. / Joubert-Caron, Raymonde; Caron, Michel; Bochet, Pascal; Chadli, Ahmed; Delaporte, Pierre; Schuller, Edmond; Bladier, Dominique.

In: International Journal of Biochemistry, Vol. 26, No. 6, 01.01.1994, p. 813-823.

Research output: Contribution to journalArticle

Joubert-Caron, Raymonde ; Caron, Michel ; Bochet, Pascal ; Chadli, Ahmed ; Delaporte, Pierre ; Schuller, Edmond ; Bladier, Dominique. / Oligoclonal β-galactoside-binding immunoglobulins antigenically related to 14kda lectin in human serum and cerebrospinal fluid : Purification and characterization. In: International Journal of Biochemistry. 1994 ; Vol. 26, No. 6. pp. 813-823.
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