Oligodendroglial cyclic AMP response element‐binding protein: A member of the CREB family of transcription factors

C. Sato‐Bigbee, E. L.P. Chan, R. K. Yu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Several laboratories have shown that cyclic AMP (cAMP) plays an important role in inducing oligodendrocyte differentiation and myelin synthesis. Our previous results have shown that oligodendrocytes contain a nuclear protein that binds to the DNA sequence TGACGTCA or cAMP response element (CRE) known to be involved in the transcriptional regulation of cAMP‐responsive genes. In this report the oligodendroglial CRE‐binding protein was further identified by using two different antibodies which specifically recognize the CRE‐binding protein known as CREB. In DNA‐shift assays CREB‐1 (X‐12) antibody interacted with the CRE‐protein complexes resulting in further retardation (“super shift”) of the mobility of the bands in the gels. Immunoprecipitation of oligodendroglial nuclear extracts with CREB(240) antibody prior to the DNA binding assays resulted in a lack of formation of CRE‐protein complexes. In addition immunoreaction with CREB(240) antibody identified the CRE‐binding species as a 45 kDa phosphoprotein. Immunocytochemical staining with CREB(240) antibody in oligodendrocytes from 10‐, 14‐, and 18‐day‐old and adult rats indicated that this protein in expressed before the appearance of myelin basic protein (MBP) which was used as a marker of myelin synthesis. Collectively, these observations support our previous results and indicate that the oligodendroglial CRE‐binding protein species is highly homologous to the CREB protein. The developmental expression of this CREB protein supports the idea of a possible role during the early stages of oligodendrocyte differentiation preceding the peak of myelin synthesis in rat CNS. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)621-628
Number of pages8
JournalJournal of Neuroscience Research
Volume38
Issue number6
DOIs
StatePublished - Aug 15 1994

Fingerprint

Staphylococcal Protein A
Cyclic AMP
Oligodendroglia
Transcription Factors
Myelin Sheath
Antibodies
Cyclic AMP Response Element-Binding Protein
Proteins
Myelin Basic Protein
Phosphoproteins
Response Elements
Nuclear Proteins
Immunoprecipitation
Gels
Staining and Labeling
DNA
Genes

Keywords

  • CREB
  • cyclic AMP
  • myelin synthesis
  • oligodendrocyte differentiation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Oligodendroglial cyclic AMP response element‐binding protein : A member of the CREB family of transcription factors. / Sato‐Bigbee, C.; Chan, E. L.P.; Yu, R. K.

In: Journal of Neuroscience Research, Vol. 38, No. 6, 15.08.1994, p. 621-628.

Research output: Contribution to journalArticle

@article{3a6fffa713ff4e16a9c6c039dbaac602,
title = "Oligodendroglial cyclic AMP response element‐binding protein: A member of the CREB family of transcription factors",
abstract = "Several laboratories have shown that cyclic AMP (cAMP) plays an important role in inducing oligodendrocyte differentiation and myelin synthesis. Our previous results have shown that oligodendrocytes contain a nuclear protein that binds to the DNA sequence TGACGTCA or cAMP response element (CRE) known to be involved in the transcriptional regulation of cAMP‐responsive genes. In this report the oligodendroglial CRE‐binding protein was further identified by using two different antibodies which specifically recognize the CRE‐binding protein known as CREB. In DNA‐shift assays CREB‐1 (X‐12) antibody interacted with the CRE‐protein complexes resulting in further retardation (“super shift”) of the mobility of the bands in the gels. Immunoprecipitation of oligodendroglial nuclear extracts with CREB(240) antibody prior to the DNA binding assays resulted in a lack of formation of CRE‐protein complexes. In addition immunoreaction with CREB(240) antibody identified the CRE‐binding species as a 45 kDa phosphoprotein. Immunocytochemical staining with CREB(240) antibody in oligodendrocytes from 10‐, 14‐, and 18‐day‐old and adult rats indicated that this protein in expressed before the appearance of myelin basic protein (MBP) which was used as a marker of myelin synthesis. Collectively, these observations support our previous results and indicate that the oligodendroglial CRE‐binding protein species is highly homologous to the CREB protein. The developmental expression of this CREB protein supports the idea of a possible role during the early stages of oligodendrocyte differentiation preceding the peak of myelin synthesis in rat CNS. {\circledC} 1994 Wiley‐Liss, Inc.",
keywords = "CREB, cyclic AMP, myelin synthesis, oligodendrocyte differentiation",
author = "C. Sato‐Bigbee and Chan, {E. L.P.} and Yu, {R. K.}",
year = "1994",
month = "8",
day = "15",
doi = "10.1002/jnr.490380604",
language = "English (US)",
volume = "38",
pages = "621--628",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Oligodendroglial cyclic AMP response element‐binding protein

T2 - A member of the CREB family of transcription factors

AU - Sato‐Bigbee, C.

AU - Chan, E. L.P.

AU - Yu, R. K.

PY - 1994/8/15

Y1 - 1994/8/15

N2 - Several laboratories have shown that cyclic AMP (cAMP) plays an important role in inducing oligodendrocyte differentiation and myelin synthesis. Our previous results have shown that oligodendrocytes contain a nuclear protein that binds to the DNA sequence TGACGTCA or cAMP response element (CRE) known to be involved in the transcriptional regulation of cAMP‐responsive genes. In this report the oligodendroglial CRE‐binding protein was further identified by using two different antibodies which specifically recognize the CRE‐binding protein known as CREB. In DNA‐shift assays CREB‐1 (X‐12) antibody interacted with the CRE‐protein complexes resulting in further retardation (“super shift”) of the mobility of the bands in the gels. Immunoprecipitation of oligodendroglial nuclear extracts with CREB(240) antibody prior to the DNA binding assays resulted in a lack of formation of CRE‐protein complexes. In addition immunoreaction with CREB(240) antibody identified the CRE‐binding species as a 45 kDa phosphoprotein. Immunocytochemical staining with CREB(240) antibody in oligodendrocytes from 10‐, 14‐, and 18‐day‐old and adult rats indicated that this protein in expressed before the appearance of myelin basic protein (MBP) which was used as a marker of myelin synthesis. Collectively, these observations support our previous results and indicate that the oligodendroglial CRE‐binding protein species is highly homologous to the CREB protein. The developmental expression of this CREB protein supports the idea of a possible role during the early stages of oligodendrocyte differentiation preceding the peak of myelin synthesis in rat CNS. © 1994 Wiley‐Liss, Inc.

AB - Several laboratories have shown that cyclic AMP (cAMP) plays an important role in inducing oligodendrocyte differentiation and myelin synthesis. Our previous results have shown that oligodendrocytes contain a nuclear protein that binds to the DNA sequence TGACGTCA or cAMP response element (CRE) known to be involved in the transcriptional regulation of cAMP‐responsive genes. In this report the oligodendroglial CRE‐binding protein was further identified by using two different antibodies which specifically recognize the CRE‐binding protein known as CREB. In DNA‐shift assays CREB‐1 (X‐12) antibody interacted with the CRE‐protein complexes resulting in further retardation (“super shift”) of the mobility of the bands in the gels. Immunoprecipitation of oligodendroglial nuclear extracts with CREB(240) antibody prior to the DNA binding assays resulted in a lack of formation of CRE‐protein complexes. In addition immunoreaction with CREB(240) antibody identified the CRE‐binding species as a 45 kDa phosphoprotein. Immunocytochemical staining with CREB(240) antibody in oligodendrocytes from 10‐, 14‐, and 18‐day‐old and adult rats indicated that this protein in expressed before the appearance of myelin basic protein (MBP) which was used as a marker of myelin synthesis. Collectively, these observations support our previous results and indicate that the oligodendroglial CRE‐binding protein species is highly homologous to the CREB protein. The developmental expression of this CREB protein supports the idea of a possible role during the early stages of oligodendrocyte differentiation preceding the peak of myelin synthesis in rat CNS. © 1994 Wiley‐Liss, Inc.

KW - CREB

KW - cyclic AMP

KW - myelin synthesis

KW - oligodendrocyte differentiation

UR - http://www.scopus.com/inward/record.url?scp=0028024621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028024621&partnerID=8YFLogxK

U2 - 10.1002/jnr.490380604

DO - 10.1002/jnr.490380604

M3 - Article

C2 - 7528813

AN - SCOPUS:0028024621

VL - 38

SP - 621

EP - 628

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 6

ER -