On-line metabolic pathway analysis based on metabolic signal flow diagram

Huidong Shi, Kazuyuki Shimizu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this work, an integrated modeling approach based on a metabolic signal flow diagram and cellular energetics was used to model the metabolic pathway analysis for the cultivation of yeast on glucose. This approach enables us to make a clear analysis of the flow direction of the carbon fluxes in the metabolic pathways as well as of the degree of activation of a particular pathway for the synthesis of biomaterials for cell growth. The analyses demonstrate that the main metabolic pathways of Saccharomyces cerevisiae change significantly during batch culture. Carbon flow direction is toward glycolysis to satisfy the increase of requirement for precursors and energy. The enzymatic activation of TCA cycle seems to always be at normal level, which may result in the overflow of ethanol due to its limited capacity. The advantage of this approach is that it adopts both virtues of the metabolic signal flow diagram and the simple network analysis method, focusing on the investigation of the flow directions of carbon fluxes and the degree of activation of a particular pathway or reaction loop. All of the variables used in the model equations were determined on-line; the information obtained from the calculated metabolic coefficients may result in a better understanding of cell physiology and help to evaluate the state of the cell culture process.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalBiotechnology and Bioengineering
Volume58
Issue number2-3
DOIs
StatePublished - May 5 1998
Externally publishedYes

Fingerprint

Metabolic Networks and Pathways
Carbon Cycle
Carbon
Chemical activation
Yeast
Fluxes
Cell Physiological Phenomena
Batch Cell Culture Techniques
Physiology
Cell growth
Biocompatible Materials
Glycolysis
Electric network analysis
Cell culture
Biomaterials
Glucose
Saccharomyces cerevisiae
Ethanol
Cell Culture Techniques
Yeasts

Keywords

  • Metabolic and energetic model
  • Metabolic engineering
  • Pathway analysis
  • Physiological state
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

On-line metabolic pathway analysis based on metabolic signal flow diagram. / Shi, Huidong; Shimizu, Kazuyuki.

In: Biotechnology and Bioengineering, Vol. 58, No. 2-3, 05.05.1998, p. 139-148.

Research output: Contribution to journalArticle

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