Abstract
The role of self-peptides bound to MHC molecules in the selection of T cells in the thymus remains controversial. Here, we have tested whether a high-abundance single class II MHC-peptide complex has a dominant effect on the repertoire of CD4+ T cells selected by low-abundance class II MHC-peptide complexes. For these studies, we have used H-2b mice that lack an invariant chain (li) (Abli-) and their transgenic variant (AbAbEpli-) that co-expresses Ab molecules covalently bound with a single peptide Ep(52-68). In these latter mice, close to 50% of all Ab molecules are occupied by Ep(52-68) peptide. Although the AbEp complex was abundantly expressed in the thymus under conditions excluding negative selection on bone marrow-derived cells, no striking quantitative difference between repertoires of TCR expressed on CD4+ T cells in Abli- and AbAbEpli- mice was noticed. Our results are consistent with the view that diverse, low-abundance self-peptides play an important role in thymic positive selection and do not support the notion that dominant, high-abundance peptides may be critical for shaping the TCR repertoire.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 67-72 |
| Number of pages | 6 |
| Journal | International Immunology |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2000 |
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Keywords
- MHC-peptide complex
- Positive selection
- T cell repertoire
ASJC Scopus subject areas
- Immunology
Cite this
On the role of high- and low-abundance class II MHC-peptide complexes in the thymic positive selection of CD4+ T cells. / Chmielowski, Bartosz; Muranski, Pawel; Kisielow, Pawel; Ignatowicz, Leszek.
In: International Immunology, Vol. 12, No. 1, 01.01.2000, p. 67-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - On the role of high- and low-abundance class II MHC-peptide complexes in the thymic positive selection of CD4+ T cells
AU - Chmielowski, Bartosz
AU - Muranski, Pawel
AU - Kisielow, Pawel
AU - Ignatowicz, Leszek
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The role of self-peptides bound to MHC molecules in the selection of T cells in the thymus remains controversial. Here, we have tested whether a high-abundance single class II MHC-peptide complex has a dominant effect on the repertoire of CD4+ T cells selected by low-abundance class II MHC-peptide complexes. For these studies, we have used H-2b mice that lack an invariant chain (li) (Abli-) and their transgenic variant (AbAbEpli-) that co-expresses Ab molecules covalently bound with a single peptide Ep(52-68). In these latter mice, close to 50% of all Ab molecules are occupied by Ep(52-68) peptide. Although the AbEp complex was abundantly expressed in the thymus under conditions excluding negative selection on bone marrow-derived cells, no striking quantitative difference between repertoires of TCR expressed on CD4+ T cells in Abli- and AbAbEpli- mice was noticed. Our results are consistent with the view that diverse, low-abundance self-peptides play an important role in thymic positive selection and do not support the notion that dominant, high-abundance peptides may be critical for shaping the TCR repertoire.
AB - The role of self-peptides bound to MHC molecules in the selection of T cells in the thymus remains controversial. Here, we have tested whether a high-abundance single class II MHC-peptide complex has a dominant effect on the repertoire of CD4+ T cells selected by low-abundance class II MHC-peptide complexes. For these studies, we have used H-2b mice that lack an invariant chain (li) (Abli-) and their transgenic variant (AbAbEpli-) that co-expresses Ab molecules covalently bound with a single peptide Ep(52-68). In these latter mice, close to 50% of all Ab molecules are occupied by Ep(52-68) peptide. Although the AbEp complex was abundantly expressed in the thymus under conditions excluding negative selection on bone marrow-derived cells, no striking quantitative difference between repertoires of TCR expressed on CD4+ T cells in Abli- and AbAbEpli- mice was noticed. Our results are consistent with the view that diverse, low-abundance self-peptides play an important role in thymic positive selection and do not support the notion that dominant, high-abundance peptides may be critical for shaping the TCR repertoire.
KW - MHC-peptide complex
KW - Positive selection
KW - T cell repertoire
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UR - http://www.scopus.com/inward/citedby.url?scp=0033976590&partnerID=8YFLogxK
U2 - 10.1093/intimm/12.1.67
DO - 10.1093/intimm/12.1.67
M3 - Article
C2 - 10607751
AN - SCOPUS:0033976590
VL - 12
SP - 67
EP - 72
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 1
ER -