On the stiffness of demineralized dentin matrices

Heonjune Ryou, Gianluca Turco, Lorenzo Breschi, Franklin Chi Meng Tay, David Henry Pashley, Dwayne Arola

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Resin bonding to dentin requires the use of self-etching primers or acid etching to decalcify the surface and expose a layer of collagen fibrils of the dentin matrix. Acid-etching reduces the stiffness of demineralized dentin from approximately 19 GPa-1 MPa, requiring that it floats in water to prevent it from collapsing during bonding procedures. Several publications show that crosslinking agents like gluteraladehyde, carbodiimide or grape seed extract can stiffen collagen and improve resin-dentin bond strength. Objective The objective was to assess a new approach for evaluating the changes in stiffness of decalcified dentin by polar solvents and a collagen cross-linker. Methods Fully demineralized dentin beams and sections of etched coronal dentin were subjected to indentation loading using a cylindrical flat indenter in water, and after treatment with ethanol or ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The stiffness was measured as a function of strain and as a function of loading rate from 1 to 50 μm/s. Results At a strain of 0.25% the elastic modulus of the fully demineralized dentin was approximately 0.20 MPa. It increased to over 0.90 MPa at strains of 1%. Exposure to ethanol caused an increase in elastic modulus of up to four times. Increasing the loading rate from 1 to 50 μm/s caused an increase in the apparent modulus of up to three times in both water and ethanol. EDC treatment caused increases in the stiffness in fully demineralized samples and in acid-etched demineralized dentin surfaces in situ. Significance Changes in the mechanical behavior of demineralized collagen matrices can be measured effectively under hydration via indentation with cylindrical flat indenters. This approach can be used for quantifying the effects of bonding treatments on the properties of decalcified dentin after acid etching, as well as to follow the loss of stiffness over time due to enzymatic degradation.

Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalDental Materials
Volume32
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Dentin
Collagen
Stiffness
Etching
Acids
Ethanol
Indentation
Water
Resins
Elastic moduli
Grape Seed Extract
Carbodiimides
Elastic Modulus
Hydration
Crosslinking
Seed
Demineralized Dentin Matrix
Degradation
Water Purification
Publications

Keywords

  • Collagen
  • Crosslinking
  • Dentin bonding agents
  • Durability
  • EDC
  • Endogenous proteinases
  • Stiffness

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Ryou, H., Turco, G., Breschi, L., Tay, F. C. M., Pashley, D. H., & Arola, D. (2016). On the stiffness of demineralized dentin matrices. Dental Materials, 32(2), 161-170. https://doi.org/10.1016/j.dental.2015.11.029

On the stiffness of demineralized dentin matrices. / Ryou, Heonjune; Turco, Gianluca; Breschi, Lorenzo; Tay, Franklin Chi Meng; Pashley, David Henry; Arola, Dwayne.

In: Dental Materials, Vol. 32, No. 2, 01.02.2016, p. 161-170.

Research output: Contribution to journalArticle

Ryou, H, Turco, G, Breschi, L, Tay, FCM, Pashley, DH & Arola, D 2016, 'On the stiffness of demineralized dentin matrices', Dental Materials, vol. 32, no. 2, pp. 161-170. https://doi.org/10.1016/j.dental.2015.11.029
Ryou H, Turco G, Breschi L, Tay FCM, Pashley DH, Arola D. On the stiffness of demineralized dentin matrices. Dental Materials. 2016 Feb 1;32(2):161-170. https://doi.org/10.1016/j.dental.2015.11.029
Ryou, Heonjune ; Turco, Gianluca ; Breschi, Lorenzo ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Arola, Dwayne. / On the stiffness of demineralized dentin matrices. In: Dental Materials. 2016 ; Vol. 32, No. 2. pp. 161-170.
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abstract = "Resin bonding to dentin requires the use of self-etching primers or acid etching to decalcify the surface and expose a layer of collagen fibrils of the dentin matrix. Acid-etching reduces the stiffness of demineralized dentin from approximately 19 GPa-1 MPa, requiring that it floats in water to prevent it from collapsing during bonding procedures. Several publications show that crosslinking agents like gluteraladehyde, carbodiimide or grape seed extract can stiffen collagen and improve resin-dentin bond strength. Objective The objective was to assess a new approach for evaluating the changes in stiffness of decalcified dentin by polar solvents and a collagen cross-linker. Methods Fully demineralized dentin beams and sections of etched coronal dentin were subjected to indentation loading using a cylindrical flat indenter in water, and after treatment with ethanol or ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The stiffness was measured as a function of strain and as a function of loading rate from 1 to 50 μm/s. Results At a strain of 0.25{\%} the elastic modulus of the fully demineralized dentin was approximately 0.20 MPa. It increased to over 0.90 MPa at strains of 1{\%}. Exposure to ethanol caused an increase in elastic modulus of up to four times. Increasing the loading rate from 1 to 50 μm/s caused an increase in the apparent modulus of up to three times in both water and ethanol. EDC treatment caused increases in the stiffness in fully demineralized samples and in acid-etched demineralized dentin surfaces in situ. Significance Changes in the mechanical behavior of demineralized collagen matrices can be measured effectively under hydration via indentation with cylindrical flat indenters. This approach can be used for quantifying the effects of bonding treatments on the properties of decalcified dentin after acid etching, as well as to follow the loss of stiffness over time due to enzymatic degradation.",
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