TY - JOUR
T1 - Oncogenic transformation of primary hamster embryo cells by equine herpesvirus type 3
AU - Sullivan, Donna C.
AU - Atherton, Sally S.
AU - Caughman, Gretchen B.
AU - Staczek, John
AU - O'Callaghan, Dennis J.
N1 - Funding Information:
We thank Cynthia Harrison and Angela Houston for typing the manuscript. We also thank Dr. John Jackson for the karyotype analyses. Support for this investigation was obtained from research grants AI 22001, A122894, and AI 21996 from the National Institutes of Health and a Grayson Foundation research grant.
PY - 1986/8
Y1 - 1986/8
N2 - Infection of nonpermissive primary hamster embryo cells with equine herpesvirus type 3 (EHV-3; multiplicity of infection = 10 pfu/cell) resulted in an abortive infection and the development of several hundred foci of rapidly growing cells. Five of these foci were chosen at random for the establishment of transformed cell lines, designated EVD-1 (equine venereal disease) through 5. These transformed cell lines exhibited altered biological properties typical of transformed cells, including immortality, growth to high saturation density, colony formation in soft agar, reduced serum requirements, aneuploid karyotype, and oncogenicity in syngeneic animals. Subsequently, five corresponding tumor cell lines (EVD-1T through 5T) with similar biological properties were established. All EHV-3 transformed and tumor cell lines have been shown to express EHV-3-specific proteins by indirect immunofluorescence assays employing rabbit antisera to EHV-3 infected equine cells. None of the transformed cell lines were found to release infectious virus by infectious center or cocultivation assay or to contain viral particles by electron microscopy.
AB - Infection of nonpermissive primary hamster embryo cells with equine herpesvirus type 3 (EHV-3; multiplicity of infection = 10 pfu/cell) resulted in an abortive infection and the development of several hundred foci of rapidly growing cells. Five of these foci were chosen at random for the establishment of transformed cell lines, designated EVD-1 (equine venereal disease) through 5. These transformed cell lines exhibited altered biological properties typical of transformed cells, including immortality, growth to high saturation density, colony formation in soft agar, reduced serum requirements, aneuploid karyotype, and oncogenicity in syngeneic animals. Subsequently, five corresponding tumor cell lines (EVD-1T through 5T) with similar biological properties were established. All EHV-3 transformed and tumor cell lines have been shown to express EHV-3-specific proteins by indirect immunofluorescence assays employing rabbit antisera to EHV-3 infected equine cells. None of the transformed cell lines were found to release infectious virus by infectious center or cocultivation assay or to contain viral particles by electron microscopy.
KW - equine herpesvirus
KW - herpesvirus oncogenesis
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U2 - 10.1016/0168-1702(86)90018-3
DO - 10.1016/0168-1702(86)90018-3
M3 - Article
C2 - 3765823
AN - SCOPUS:0022483698
SN - 0168-1702
VL - 5
SP - 201
EP - 212
JO - Virus Research
JF - Virus Research
IS - 2-3
ER -