Oncogenic transformation of primary hamster embryo cells by equine herpesvirus type 3

Donna C. Sullivan, Sally S. Atherton, Gretchen B Caughman, John Staczek, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Infection of nonpermissive primary hamster embryo cells with equine herpesvirus type 3 (EHV-3; multiplicity of infection = 10 pfu/cell) resulted in an abortive infection and the development of several hundred foci of rapidly growing cells. Five of these foci were chosen at random for the establishment of transformed cell lines, designated EVD-1 (equine venereal disease) through 5. These transformed cell lines exhibited altered biological properties typical of transformed cells, including immortality, growth to high saturation density, colony formation in soft agar, reduced serum requirements, aneuploid karyotype, and oncogenicity in syngeneic animals. Subsequently, five corresponding tumor cell lines (EVD-1T through 5T) with similar biological properties were established. All EHV-3 transformed and tumor cell lines have been shown to express EHV-3-specific proteins by indirect immunofluorescence assays employing rabbit antisera to EHV-3 infected equine cells. None of the transformed cell lines were found to release infectious virus by infectious center or cocultivation assay or to contain viral particles by electron microscopy.

Original languageEnglish (US)
Pages (from-to)201-212
Number of pages12
JournalVirus Research
Volume5
Issue number2-3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Equid Herpesvirus 3
Transformed Cell Line
Cricetinae
Embryonic Structures
Tumor Cell Line
Infection
Horse Diseases
Virus Release
Aneuploidy
Indirect Fluorescent Antibody Technique
Sexually Transmitted Diseases
Coculture Techniques
Karyotype
Virion
Horses
Agar
Immune Sera
Electron Microscopy
Rabbits
Growth

Keywords

  • equine herpesvirus
  • herpesvirus oncogenesis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Oncogenic transformation of primary hamster embryo cells by equine herpesvirus type 3. / Sullivan, Donna C.; Atherton, Sally S.; Caughman, Gretchen B; Staczek, John; O'Callaghan, Dennis J.

In: Virus Research, Vol. 5, No. 2-3, 01.01.1986, p. 201-212.

Research output: Contribution to journalArticle

Sullivan, Donna C. ; Atherton, Sally S. ; Caughman, Gretchen B ; Staczek, John ; O'Callaghan, Dennis J. / Oncogenic transformation of primary hamster embryo cells by equine herpesvirus type 3. In: Virus Research. 1986 ; Vol. 5, No. 2-3. pp. 201-212.
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