Optimization of cryoprotectant loading into murine and human oocytes

Jens O.M. Karlsson, Edyta A. Szurek, Adam Z. Higgins, Sang R. Lee, Ali Eroglu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity. Thus, we investigated the use of physics-based mathematical optimization to guide design of cryoprotectant loading methods for mouse and human oocytes. We first examined loading of 1.5M dimethyl sulfoxide (Me2SO) into mouse oocytes at 23°C. Conventional one-step loading resulted in rates of fertilization (34%) and embryonic development (60%) that were significantly lower than those of untreated controls (95% and 94%, respectively). In contrast, the mathematically optimized two-step method yielded much higher rates of fertilization (85%) and development (87%). To examine the causes for oocyte damage, we performed experiments to separate the effects of cell shrinkage and Me2SO exposure time, revealing that neither shrinkage nor Me2SO exposure single-handedly impairs the fertilization and development rates. Thus, damage during one-step Me2SO addition appears to result from interactions between the effects of Me2SO toxicity and osmotic stress. We also investigated Me2SO loading into mouse oocytes at 30°C. At this temperature, fertilization rates were again lower after one-step loading (8%) in comparison to mathematically optimized two-step loading (86%) and untreated controls (96%). Furthermore, our computer algorithm generated an effective strategy for reducing Me2SO exposure time, using hypotonic diluents for cryoprotectant solutions. With this technique, 1.5M Me2SO was successfully loaded in only 2.5min, with 92% fertilizability. Based on these promising results, we propose new methods to load cryoprotectants into human oocytes, designed using our mathematical optimization approach.

Original languageEnglish (US)
Pages (from-to)18-28
Number of pages11
JournalCryobiology
Volume68
Issue number1
DOIs
StatePublished - Feb 1 2014

Fingerprint

cryoprotectants
Oocytes
oocytes
Fertilization
mice
Toxicity
Osmotic Pressure
osmotic stress
shrinkage
exposure duration
Dimethyl Sulfoxide
toxicity
Physics
Cryopreservation
physics
dimethyl sulfoxide
methodology
cryopreservation
Embryonic Development
embryogenesis

Keywords

  • Cryopreservation
  • Cryoprotectant
  • DMSO
  • Dimethyl sulfoxide
  • Freezing
  • Human
  • Oocyte
  • Propylene glycol
  • Simplex optimization
  • Vitrification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Optimization of cryoprotectant loading into murine and human oocytes. / Karlsson, Jens O.M.; Szurek, Edyta A.; Higgins, Adam Z.; Lee, Sang R.; Eroglu, Ali.

In: Cryobiology, Vol. 68, No. 1, 01.02.2014, p. 18-28.

Research output: Contribution to journalArticle

Karlsson, Jens O.M. ; Szurek, Edyta A. ; Higgins, Adam Z. ; Lee, Sang R. ; Eroglu, Ali. / Optimization of cryoprotectant loading into murine and human oocytes. In: Cryobiology. 2014 ; Vol. 68, No. 1. pp. 18-28.
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